A critical analysis of L-asparaginase activity quantification methods—colorimetric methods versus high-performance liquid chromatography
Sign Up to like & getrecommendations! Published in 2018 at "Analytical and Bioanalytical Chemistry"
DOI: 10.1007/s00216-018-1326-x
Abstract: AbstractL-asparaginase or ASNase (L-asparagine aminohydrolase, E.C.3.5.1.1) is an enzyme clinically accepted as an antitumor agent to treat acute lymphoblastic leukemia (ALL) and lymphosarcoma through the depletion of L-asparagine (L-Asn) resulting in cytotoxicity to leukemic cells. ASNase… read more here.
Keywords: method; colorimetric methods; asnase; quantification ... See more keywords
Chloro-Modified Magnetic Fe3O4@MCM-41 Core–Shell Nanoparticles for L-Asparaginase Immobilization with Improved Catalytic Activity, Reusability, and Storage Stability
Sign Up to like & getrecommendations! Published in 2018 at "Applied Biochemistry and Biotechnology"
DOI: 10.1007/s12010-018-2853-9
Abstract: This paper describes a new support that permits to efficient immobilization of L-asparaginase (L-ASNase). For this purpose, Fe3O4 magnetic nanoparticles were synthesized and coated by MCM-41. 3-chloropropyltrimethoxysilane (CPTMS) was used as a surface modifying agent… read more here.
Keywords: fe3o4 mcm; immobilization; reusability storage; asnase ... See more keywords
Characterization of novel L-asparaginases having clinically safe profiles from bacteria inhabiting the hemolymph of the crab, Scylla serrata (Forskål, 1775).
Sign Up to like & getrecommendations! Published in 2022 at "Folia microbiologica"
DOI: 10.1007/s12223-022-00952-x
Abstract: L-asparaginase (ASNase) is the principal chemotherapeutic agent against different blood cancers. The risks associated with current clinical preparations demand screening for novel ASNases. Accordingly, the study was conducted to shortlist ASNases having clinically safer profiles… read more here.
Keywords: characterization novel; hemolymph; asnase; step ... See more keywords
Glycosylation of Erwinase results in active protein less recognized by antibodies
Sign Up to like & getrecommendations! Published in 2020 at "Biochemical Engineering Journal"
DOI: 10.1016/j.bej.2020.107750
Abstract: Abstract L-asparaginase (L-ASNase) is an important bacterial enzyme used as a biopharmaceutical to treat acute lymphoblastic leukemia (ALL). Side effects of L–ASNase therapy have been counteracted by polyethylene glycol (PEG)-modification of L-ASNase; however, immunogenicity of… read more here.
Keywords: erwinase results; glycosylation; results active; asnase ... See more keywords
Maltose Functionalized Magnetic Core/Shell Fe3O4@Au Nanoparticles for An Efficient L-Asparaginase Immobilization.
Sign Up to like & getrecommendations! Published in 2019 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2019.09.116
Abstract: In this study, maltose-functionalized magnetic core/shell nanoparticles (Fe3O4@Au NPs) as a promising carrier matrix for a simple and effective immobilization of L-asparaginase (L-ASNase) were prepared and characterized using imaging techniques including atomic force microscopy (AFM)… read more here.
Keywords: magnetic core; core shell; microscopy; functionalized magnetic ... See more keywords
Implementation of the asparaginase activity assessment technique for clinical use: experience of a Brazilian Center
Sign Up to like & getrecommendations! Published in 2020 at "Scientific Reports"
DOI: 10.1038/s41598-020-78549-y
Abstract: Acute lymphoid leukemia is a childhood cancer that in high-income countries has event-free survival rates of 80% and global survival rates of 90%. In Brazil these rates are under 70%. This difference may be due… read more here.
Keywords: technique; implementation asparaginase; asnase activity; asnase ... See more keywords
Sequencing and characterization of an L-asparaginase gene from a new species of Penicillium section Citrina isolated from Cerrado
Sign Up to like & getrecommendations! Published in 2021 at "Scientific Reports"
DOI: 10.1038/s41598-021-97316-1
Abstract: The enzyme L-asparaginase (L-ASNase) is used in the treatment of Acute Lymphoblastic Leukemia. The preparations of this enzyme for clinical use are derived from bacterial sources and its use is associated with serious adverse reactions.… read more here.
Keywords: sequencing characterization; new species; asparaginase gene; asnase ... See more keywords
Magnetic-propelled Fe3O4–chitosan carriers enhance L-asparaginase catalytic activity: a promising strategy for enzyme immobilization
Sign Up to like & getrecommendations! Published in 2018 at "RSC Advances"
DOI: 10.1039/c8ra06346j
Abstract: Magnetic-propelled carriers comprising magnetic Fe3O4–chitosan nanoparticles were immobilized with L-asparaginase (L-ASNase). The enzyme displayed enhanced catalytic activity in a weak magnetic field, and thermal and pH stabilities. The conjugated L-ASNase presented higher thermostability and wider… read more here.
Keywords: magnetic propelled; fe3o4 chitosan; conjugated asnase; asnase ... See more keywords
Preparation and characterization of amino and carboxyl functionalized core-shell Fe3O4/SiO2 for L-asparaginase immobilization: A comparison study
Sign Up to like & getrecommendations! Published in 2020 at "Biocatalysis and Biotransformation"
DOI: 10.1080/10242422.2020.1767605
Abstract: Abstract Magnetic nanoparticles are well known as facile and effective support for enzyme immobilization since they have a high surface area, large surface-to-volume ratio, easy separation, a fast and high enzyme loading. This study aims… read more here.
Keywords: immobilization; sio2 nh2; fe3o4 sio2; sio2 ... See more keywords
Prospective, real‐time monitoring of pegylated Escherichia coli and Erwinia asparaginase therapy in childhood acute lymphoblastic leukaemia and non‐Hodgkin lymphoma in Belgium
Sign Up to like & getrecommendations! Published in 2020 at "British Journal of Haematology"
DOI: 10.1111/bjh.16495
Abstract: Asparaginase (ASNase) is an important anti‐leukaemic drug in the treatment of childhood acute lymphoblastic leukaemia (ALL) and non‐Hodgkin lymphoma (NHL). A substantial proportion of patients develop hypersensitivity reactions with anti‐ASNase neutralising antibodies, resulting in allergic… read more here.
Keywords: childhood acute; erwinia; lymphoblastic leukaemia; acute lymphoblastic ... See more keywords
Activity and toxicity of intramuscular 1000 iu/m2 polyethylene glycol‐E. coli L‐asparaginase in the UKALL 2003 and UKALL 2011 clinical trials
Sign Up to like & getrecommendations! Published in 2022 at "British Journal of Haematology"
DOI: 10.1111/bjh.18158
Abstract: In successive UK clinical trials (UKALL 2003, UKALL 2011) for paediatric acute lymphoblastic leukaemia (ALL), polyethylene glycol‐conjugated E. coli L‐asparaginase (PEG‐EcASNase) 1000 iu/m2 was administered intramuscularly with risk‐stratified treatment. In induction, patients received two PEG‐EcASNase… read more here.
Keywords: ukall 2011; ukall 2003; asnase; asparaginase ... See more keywords