Assessment of a Genomic Assay in Patients With ERBB2-Positive Breast Cancer Following Neoadjuvant Trastuzumab-Based Chemotherapy With or Without Pertuzumab.
Sign Up to like & getrecommendations! Published in 2023 at "JAMA oncology"
DOI: 10.1001/jamaoncol.2023.0187
Abstract: Importance Biomarkers to guide the use of pertuzumab in the treatment of early-stage ERBB2 (formerly HER2)-positive breast cancer beyond simple ERBB2 status are needed. Objective To determine if use of the HER2DX genomic assay (Reveal… read more here.
Keywords: assay; assay reported; breast cancer; pcr ... See more keywords
Development of a genotype‐by‐sequencing immunogenetic assay as exemplified by screening for variation in red fox with and without endemic rabies exposure
Sign Up to like & getrecommendations! Published in 2018 at "Ecology and Evolution"
DOI: 10.1002/ece3.3583
Abstract: Abstract Pathogens are recognized as major drivers of local adaptation in wildlife systems. By determining which gene variants are favored in local interactions among populations with and without disease, spatially explicit adaptive responses to pathogens… read more here.
Keywords: variation; without endemic; disease; genotype sequencing ... See more keywords
Performance evaluation of a laboratory‐developed light‐initiated chemiluminescence assay for quantification of egg white‐specific IgE
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Clinical Laboratory Analysis"
DOI: 10.1002/jcla.24544
Abstract: Specific IgE (sIgE) testing has become one of the most important tools for diagnosing IgE‐mediated food allergy. Enzyme‐linked immunosorbent assay (ELISA) and dot‐enzyme‐linked immunosorbent assay (Dot‐ELISA) have been used to measure sIgE in clinical widely.… read more here.
Keywords: assay; specific ige; initiated chemiluminescence; ige ... See more keywords
A yeast‐based complementation assay elucidates the functional impact of 200 missense variants in human PSAT1
Sign Up to like & getrecommendations! Published in 2020 at "Journal of Inherited Metabolic Disease"
DOI: 10.1002/jimd.12227
Abstract: Defects in serine biosynthesis resulting from loss of function mutations in PHGDH, PSAT1, and PSPH cause a set of rare, autosomal recessive diseases known as Neu‐Laxova syndrome (NLS) or serine‐deficiency disorders. The diseases present with… read more here.
Keywords: yeast based; functional impact; assay; psat1 ... See more keywords
One‐step quantitative RT‐PCR assay with armored RNA controls for detection of SARS‐CoV‐2
Sign Up to like & getrecommendations! Published in 2020 at "Journal of Medical Virology"
DOI: 10.1002/jmv.26540
Abstract: Coronavirus disease 2019 (COVID‐19) has become pandemic since March 11, 2020. Thus, development and integration in clinics of fast and sensitive diagnostic tools are essential. The aim of the study is a development and evaluation… read more here.
Keywords: covid amp; detection; step quantitative; assay ... See more keywords
Determining Phosphodiesterase Activity (Radioactive Assay).
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7240-1_21
Abstract: Cyclic-di-GMP phosphodiesterases (PDEs) catalyze the hydrolysis of the bacterial second messenger c-di-GMP. This protocol describes a sensitive radioactive assay for PDE activity in which substrate and product can be quickly and easily separated by thin-layer… read more here.
Keywords: radioactive assay; determining phosphodiesterase; assay; activity radioactive ... See more keywords
Integrating high-performing electrochemical transducers in lateral flow assay
Sign Up to like & getrecommendations! Published in 2021 at "Analytical and Bioanalytical Chemistry"
DOI: 10.1007/s00216-021-03301-y
Abstract: Lateral flow assays (LFAs) are the best-performing and best-known point-of-care tests worldwide. Over the last decade, they have experienced an increasing interest by researchers towards improving their analytical performance while maintaining their robust assay platform.… read more here.
Keywords: high performing; assay; integrating high; performance ... See more keywords
A simple enzymatic assay for the quantification of C1-specific cellulose oxidation by lytic polysaccharide monooxygenases
Sign Up to like & getrecommendations! Published in 2019 at "Biotechnology Letters"
DOI: 10.1007/s10529-019-02760-9
Abstract: Objective The development of an enzymatic assay for the specific quantification of the C1-oxidation product, i.e. gluconic acid of cellulose active lytic polysaccharide monooxygenases (LPMOs). Results In combination with a β-glucosidase, the spectrophotometrical assay can… read more here.
Keywords: oxidation; enzymatic assay; assay; polysaccharide monooxygenases ... See more keywords
Identification of human flavin-containing monooxygenase 3 substrates by a colorimetric screening assay.
Sign Up to like & getrecommendations! Published in 2017 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2017.01.024
Abstract: Human hepatic flavin-containing monooxygenase 3 is a phase I drug-metabolizing enzyme that is responsible for the oxidation of a variety of drugs and xenobiotics. This work reports on a high throughput rapid colorimetric assay for… read more here.
Keywords: containing monooxygenase; identification human; flavin containing; assay ... See more keywords
Quantitative assay of β-(1,3)-β-(1,6)-glucans from fermentation broth using aniline blue.
Sign Up to like & getrecommendations! Published in 2017 at "Carbohydrate polymers"
DOI: 10.1016/j.carbpol.2017.06.047
Abstract: An aniline blue fluorescence assay was constructed to quantify two fungal β-(1,3)-glucans with β-(1,6)-sidechains: scleroglucan and schizophyllan. Calibration curves were linear in the range from 30mg/l to 6g/l with R2>99.8%. The assay was robust against… read more here.
Keywords: glucans fermentation; quantitative assay; assay; assay glucans ... See more keywords
A Scalable, Multiplexed Assay for Decoding GPCR-Ligand Interactions with RNA Sequencing.
Sign Up to like & getrecommendations! Published in 2019 at "Cell systems"
DOI: 10.1016/j.cels.2019.02.009
Abstract: G protein-coupled receptors (GPCRs) are central to how mammalian cells sense and respond to chemicals. Mammalian olfactory receptors (ORs), the largest family of GPCRs, mediate the sense of smell through activation by small molecules, though… read more here.
Keywords: multiplexed assay; scalable multiplexed; decoding gpcr; assay decoding ... See more keywords