A Cell-Based Fluorescent Assay to Detect the Activity of AB Toxins that Inhibit Protein Synthesis.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6958-6_3
Abstract: Many AB toxins elicit a cytotoxic effect involving the inhibition of protein synthesis. In this chapter, we describe a simple cell-based fluorescent assay to detect and quantify the inhibition of protein synthesis. The assay can… read more here.
Keywords: fluorescent assay; cell based; protein synthesis; based fluorescent ... See more keywords
A Fluorescent Reverse-Transcription Assay to Detect Chemical Adducts on RNA.
Sign Up to like & getrecommendations! Published in 2022 at "Biochemistry"
DOI: 10.1021/acs.biochem.2c00270
Abstract: Herein, we detail a novel reverse-transcription (RT) assay to directly detect chemical adducts on RNA. We optimize a fluorescence quenching assay to detect RT polymerization and employ our approach to detect N1-alkylation of inosine, an… read more here.
Keywords: assay detect; adducts rna; detect chemical; transcription assay ... See more keywords
A serological assay to detect SARS-CoV-2 seroconversion in humans
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DOI: 10.1038/s41591-020-0913-5
Abstract: Here, we describe a serological enzyme-linked immunosorbent assay for the screening and identification of human SARS-CoV-2 seroconverters. This assay does not require the handling of infectious virus, can be adjusted to detect different antibody types… read more here.
Keywords: detect sars; seroconversion humans; cov seroconversion; sars cov ... See more keywords
The update and optimization of an eDNA assay to detect the invasive rusty crayfish (Faxonius rusticus)
Sign Up to like & getrecommendations! Published in 2021 at "PLoS ONE"
DOI: 10.1371/journal.pone.0259084
Abstract: Environmental DNA (eDNA) is nuclear or mitochondrial DNA shed into the environment, and amplifying this DNA can serve as a reliable, noninvasive way to monitor aquatic systems for the presence of an invasive species. Assays… read more here.
Keywords: invasive rusty; detect invasive; dna; rusticus ... See more keywords