An Ultrasensitive Fluorescence Assay for the Detection of Halides and Enzymatic Dehalogenation
Sign Up to like & getrecommendations! Published in 2020 at "Chemcatchem"
DOI: 10.1002/cctc.201901891
Abstract: Halide assays are important for the study of enzymatic dehalogenation, a topic of great industrial and scientific importance. Here we describe the development of a very sensitive halide assay that can detect less than a… read more here.
Keywords: dehalogenation; assay detection; ultrasensitive fluorescence; enzymatic dehalogenation ... See more keywords
Evaluate performance of the Abbott chemiluminescent microparticle immunoassay assay for detection of syphilis infection in Chinese blood donors
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Clinical Laboratory Analysis"
DOI: 10.1002/jcla.23033
Abstract: To prevent Treponema Pallidum (TP) transmission from blood transfusion, enzyme‐linked immunosorbent assay (EIA) for anti‐TP has been widely used in routine blood donation screening in China for many years. The aim of this study was… read more here.
Keywords: assay detection; chinese blood; blood donors; performance abbott ... See more keywords
Optimisation of a high-throughput fluorescamine assay for detection of N-acyl-l-homoserine lactone acylase activity.
Sign Up to like & getrecommendations! Published in 2019 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2018.10.029
Abstract: N-acyl-l-homoserine lactone (AHL) acylases are a well-known group of enzymes that disrupt quorum sensing in Gram-negative bacteria by degrading AHL signalling molecules. This degradation of signalling molecules (termed 'quorum quenching') has potential uses in the… read more here.
Keywords: assay detection; acyl homoserine; homoserine lactone; acylase activity ... See more keywords
A seminested PCR assay for detection and typing of human papillomavirus based on E1 gene sequences.
Sign Up to like & getrecommendations! Published in 2018 at "Diagnostic microbiology and infectious disease"
DOI: 10.1016/j.diagmicrobio.2017.12.016
Abstract: HPV infection is considered one of the leading causes of cervical cancer in the world. To date, more than 180 types of HPV have been described and viral typing is critical for defining the prognosis… read more here.
Keywords: assay detection; detection typing; pcr assay; seminested pcr ... See more keywords
A 'light-up' intercalator displacement assay for detection of triplex DNA stabilizers.
Sign Up to like & getrecommendations! Published in 2020 at "Chemical communications"
DOI: 10.1039/c9cc08817b
Abstract: Here, we developed a coralyne-based, 'light-up' intercalator displacement assay to identify molecular stabilizers of triplex DNA using a sequence from a chromosomal breakpoint hotspot in the human c-MYC oncogene. Its potential to identify triplex DNA… read more here.
Keywords: intercalator displacement; displacement assay; triplex dna; assay detection ... See more keywords
ClonoSEQ assay for the detection of lymphoid malignancies
Sign Up to like & getrecommendations! Published in 2019 at "Expert Review of Molecular Diagnostics"
DOI: 10.1080/14737159.2019.1627877
Abstract: ABSTRACT Introduction: Immunoglobulin rearrangement studies by molecular methods are routinely used to detect clonality and to follow up patients with lymphoid malignancies. The design of a next-generation sequencing (NGS) panel using a comprehensive pool of… read more here.
Keywords: detection; assay detection; detection lymphoid; review ... See more keywords
A Multianalyte Assay for the Detection of Dermatomyositis-Related Autoantibodies Based on Immunoprecipitation Combined With Immunoblotting.
Sign Up to like & getrecommendations! Published in 2022 at "Modern rheumatology"
DOI: 10.1093/mr/roac056
Abstract: OBJECTIVE To develop a multianalyte assay for the detection of dermatomyositis (DM)-related autoantibodies using immunoprecipitation (IP) combined with immunoblotting (IB). METHODS Sera from 116 DM patients were subjected to RNA and protein immunoprecipitation assays as… read more here.
Keywords: assay detection; related autoantibodies; anti; multianalyte assay ... See more keywords
A qPCR Assay for the Detection of Phytophthora cinnamomi Including an mRNA Protocol Designed to Establish Propagule Viability in Environmental Samples.
Sign Up to like & getrecommendations! Published in 2019 at "Plant disease"
DOI: 10.1094/pdis-09-18-1641-re
Abstract: Phytophthora cinnamomi causes root and collar rot in many plant species in natural ecosystems and horticulture. A species-specific primer and probe PCIN5 were designed based on a mitochondrial locus encoding subunit 2 of cytochrome c… read more here.
Keywords: qpcr assay; environmental samples; assay detection; protocol ... See more keywords
Development of a quantitative PCR assay for detection of redside shiner (Richardsonius balteatus) from environmental DNA
Sign Up to like & getrecommendations! Published in 2019 at "BMC Research Notes"
DOI: 10.1186/s13104-019-4819-6
Abstract: ObjectiveA quantitative PCR (qPCR) assay for the detection of redside shiner (Richardsonius balteatus) environmental DNA (eDNA) was designed as a side product of a larger project aimed at using eDNA to determine the presence and… read more here.
Keywords: quantitative pcr; assay detection; dna; redside shiner ... See more keywords
Easyscreen™ Enteric Protozoa Assay for the Detection of Intestinal Parasites: A Retrospective Bi-Center Study
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Parasitology"
DOI: 10.1645/18-52
Abstract: ABSTRACT Gastroenteritis caused by single or multiple pathogens remains a major diagnostic challenge for the laboratory, as diagnosis is achieved using different techniques with variable sensitivity and specificity. The aim of this study was to… read more here.
Keywords: detection; assay detection; easyscreen enteric; study ... See more keywords
A cell-based assay for the detection of neutralizing antibodies against alemtuzumab
Sign Up to like & getrecommendations! Published in 2020 at "Biotechniques"
DOI: 10.2144/btn-2019-0122
Abstract: Aim: The humanized anti-CD52 monoclonal antibody alemtuzumab depletes lymphocytes and is currently used to treat relapsing multiple sclerosis. During treatment, anti-alemtuzumab antibodies may develop and reduce effective lymphocyte depletion in future treatment cycles. Results: Alemtuzumab–Alexa… read more here.
Keywords: anti alemtuzumab; assay detection; cell based; neutralizing antibodies ... See more keywords