Fluorescence-Based Assay For Measuring OMA1 Activity.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1266-8_24
Abstract: Mitochondrial fusion depends on proteolytic processing of the dynamin-related GTPase protein, OPA1, which is regulated by the mitochondrial zinc metalloproteinase, OMA1. Last year we published a report describing a novel approach to directly measure the… read more here.
Keywords: fluorescence based; oma1 activity; assay measuring; based assay ... See more keywords
Plate-Based Assay for Measuring Direct Semaphorin-Neuropilin Interactions.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6448-2_5
Abstract: The semaphorins are an essential family of axon guidance molecules that can be either secreted or are transmembrane proteins. Class 3 semaphorin (Sema3) family members are secreted and provide long-range guidance cues through two receptor… read more here.
Keywords: semaphorin; sema3 nrp; plate based; assay measuring ... See more keywords
An Assay for Measuring Histone Variant Exchange within Nucleosomes In Vitro.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6630-1_2
Abstract: The incorporation of histone variants into specific chromatin regions is a mechanism by which cells can regulate many important biological processes. One such example is H2A.Z, a highly conserved variant of H2A that is incorporated… read more here.
Keywords: vitro assay; variant exchange; h2a; exchange ... See more keywords
CUPRA-ZYME: An assay for measuring carbohydrate-active enzyme activities, pathways and substrate specificities.
Sign Up to like & getrecommendations! Published in 2020 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.9b05007
Abstract: Carbohydrate-Active enZymes (CAZymes) are involved in the synthesis, degradation and modification of carbohydrates. They play critical roles in diverse physiological and pathophysiological processes, have important industrial and biotechnological applications, are important drug targets and represent… read more here.
Keywords: pathways substrate; cupra zyme; substrate specificities; assay measuring ... See more keywords
Quantitative bioluminescence assay for measuring Bacillus cereus nonhemolytic enterotoxin complex
Sign Up to like & getrecommendations! Published in 2020 at "PLoS ONE"
DOI: 10.1371/journal.pone.0238153
Abstract: Bacillus cereus is a foodborne pathogen causing emesis and diarrhea in those affected. It is assumed that the non-hemolytic enterotoxin (Nhe) plays a key role in B. cereus induced diarrhea. The ability to trace Nhe… read more here.
Keywords: bacillus cereus; quantitative bioluminescence; enterotoxin; bioluminescence assay ... See more keywords
A Direct Assay for Measuring Free 25-Hydroxyvitamin D.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of AOAC International"
DOI: 10.5740/jaoacint.17-0084
Abstract: Recent studies suggest that the concentration and genotype of vitamin D binding protein (VDBP) are important factors that determine the bioavailability of 25-hydroxyvitamin D [25(OH)D] in blood. Accumulating data indicate that, e.g., in pregnant women,… read more here.
Keywords: hydroxyvitamin; free hydroxyvitamin; direct assay; measuring free ... See more keywords