Versatile High-Throughput Fluorescence Assay for Monitoring Cas9 Activity.
Sign Up to like & getrecommendations! Published in 2018 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.8b01155
Abstract: The RNA-guided DNA nuclease Cas9 is now widely used for the targeted modification of genomes of human cells and various organisms. Despite the extensive use of Clustered Regularly Interspaced Palindromic Repeats (CRISPR) systems for genome… read more here.
Keywords: activity; high throughput; fluorescence assay; cas9 ... See more keywords
Application of anti-Xa assay in monitoring unfractionated heparin therapy in contemporary antithrombotic management
Sign Up to like & getrecommendations! Published in 2023 at "Expert Review of Hematology"
DOI: 10.1080/17474086.2023.2169126
Abstract: ABSTRACT Introduction Unfractionated heparin remains the most widely used agent in the prevention and acute treatment of thrombosis. Pharmacological complexities of this intriguing agent mandate frequent monitoring of its anticoagulant properties to maintain safe and… read more here.
Keywords: management; anti assay; assay monitoring; unfractionated heparin ... See more keywords
A molecular beacon assay for monitoring RNA splicing
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac242
Abstract: Abstract Small molecule targeting of self-splicing RNAs like group I and II introns has been limited in part by the lack of a universal high-throughput screening platform for studies of splicing inhibition and kinetics. Here,… read more here.
Keywords: rna splicing; beacon; monitoring rna; beacon assay ... See more keywords
Monitoring of heparin therapy beyond the anti‐Xa activity assay: Evaluation of a thrombin generation assay
Sign Up to like & getrecommendations! Published in 2022 at "International Journal of Laboratory Hematology"
DOI: 10.1111/ijlh.13836
Abstract: Global coagulation assays may be of added value to the anti‐Xa assay for monitoring heparin therapy. Unlike most testing methods, the thrombin generation assay (TGA) has the ability to assess the overall function of the… read more here.
Keywords: heparin therapy; monitoring heparin; assay monitoring; thrombin generation ... See more keywords