Luminescent Assay for the Screening of SARS‐CoV‐2 MPro Inhibitors
Sign Up to like & getrecommendations! Published in 2022 at "Chembiochem"
DOI: 10.1002/cbic.202200190
Abstract: Since the outbreak of SARS‐CoV‐2 in December 2019 millions of infections have been reported globally. The viral chymotrypsin‐like main protease (MPro) exhibits a crucial role in viral replication and represents a relevant target for antiviral… read more here.
Keywords: assay screening; screening sars; cov mpro; luminescent assay ... See more keywords
Solid-Phase Agar Plate Assay for Screening Amine Transaminases.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7366-8_17
Abstract: Agar plate assays represent a useful method for high-throughput prescreening of larger enzyme libraries derived from for example error-prone PCR or multiple site-saturation mutagenesis to decrease screening effort by separating promising variants from less active,… read more here.
Keywords: plate assay; agar; solid phase; assay screening ... See more keywords
A multiplexable assay for screening antibiotic lethality against drug-tolerant bacteria
Sign Up to like & getrecommendations! Published in 2019 at "Nature Methods"
DOI: 10.1038/s41592-019-0333-y
Abstract: Antibiotic screens typically rely on growth inhibition to characterize compound bioactivity—an approach that cannot be used to assess the bactericidal activity of antibiotics against bacteria in drug-tolerant states. To address this limitation, we developed a… read more here.
Keywords: tolerant bacteria; multiplexable assay; tolerant; drug tolerant ... See more keywords
A Straightforward Assay for Screening and Quantification of Biosurfactants in Microbial Culture Supernatants
Sign Up to like & getrecommendations! Published in 2020 at "Frontiers in Bioengineering and Biotechnology"
DOI: 10.3389/fbioe.2020.00958
Abstract: A large variety of microorganisms produces biosurfactants with the potential for a number of diverse industrial applications. To identify suitable wild-type or engineered production strains, efficient screening methods are needed, allowing for rapid and reliable… read more here.
Keywords: straightforward assay; culture supernatants; quantification biosurfactants; assay screening ... See more keywords