A substrate for a cell free in vitro assay system to screen drugs targeting trypsin like protease‐based cleavage of SARS‐CoV‐2 spike glycoprotein and viral entry
Sign Up to like & getrecommendations! Published in 2023 at "Journal of Medical Virology"
DOI: 10.1002/jmv.28796
Abstract: Host proteases trypsin and trypsin‐like proteases have been reported to facilitate the entry of coronavirus SARS‐CoV‐2 in its host cells. These protease enzymes cleave the viral surface glycoprotein, spike, leading to successful cell surface receptor… read more here.
Keywords: trypsin; cleavage; substrate; trypsin like ... See more keywords
Antibacterial activity of 1,2-dicarbonyl compounds and the influence of the in vitro assay system.
Sign Up to like & getrecommendations! Published in 2019 at "Food chemistry"
DOI: 10.1016/j.foodchem.2019.125905
Abstract: The antibacterial activities of the dicarbonyl compounds glyoxal (GO), methylglyoxal (MGO), 3-deoxyglucosone (3-DG) were assessed against Gram-positive and Gram-negative pathogenic and food spoilage bacteria, both in agarised and liquid assay system. The kinetics of dicarbonyls'… read more here.
Keywords: antibacterial activity; dicarbonyl compounds; system; assay system ... See more keywords
Rapid, Highly Sensitive, and Label-Free Pathogen Assay System Using a Solid-Phase Self-Interference Recombinase Polymerase Amplification Chip and Hyperspectral Interferometry.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.1c04858
Abstract: Recombinase polymerase amplification (RPA) is a useful pathogen identification method. Several label-free detection methods for RPA amplicons have been developed in recent years. However, these methods still lack sensitivity, specificity, efficiency, or simplicity. In this… read more here.
Keywords: system; label free; assay system; hyperspectral interferometry ... See more keywords
Development of a NanoBRET-Based Sensitive Screening Method for CXCR4 Ligands.
Sign Up to like & getrecommendations! Published in 2019 at "Bioconjugate chemistry"
DOI: 10.1021/acs.bioconjchem.9b00182
Abstract: A critical part of the development of CXCR4 modulators is to have a simple and sensitive assay system to complement the search by screening and evaluating the binding affinity. Herein, a NanoBRET assay system was… read more here.
Keywords: nanobret assay; assay system; cxcr4 ligands; development ... See more keywords
Establishment of Cell-Based Assay System for Evaluating Cytotoxic Activity Modulated by the Blockade of PD-1 and PD-L1 Interactions with a Therapeutic Antibody
Sign Up to like & getrecommendations! Published in 2023 at "Immunological Investigations"
DOI: 10.1080/08820139.2023.2174442
Abstract: ABSTRACT Background Therapeutic antibodies targeting the PD-1/PD-L1 immune checkpoint are widely used in cancer therapy and are under active further development. Historically, the antitumor activity of PD-1/PD-L1 immune checkpoint inhibitors has been evaluated using in… read more here.
Keywords: system; cell; cytotoxic activity; assay system ... See more keywords
ASO Author Reflections: New Assay System to Detect Serum Anti-p53 Antibodies
Sign Up to like & getrecommendations! Published in 2020 at "Annals of Surgical Oncology"
DOI: 10.1245/s10434-020-09360-2
Abstract: Previous studies have shown that serum anti-p53 antibodies (s-p53-Abs) can be detected independently from conventional tumor markers and may be used in diagnosis and screening, and to monitor treatment response and tumor recurrence, in patients… read more here.
Keywords: p53 abs; p53 antibodies; anti p53; assay system ... See more keywords
Assay System for Simultaneous Detection of SARS-CoV-2 and Other Respiratory Viruses
Sign Up to like & getrecommendations! Published in 2021 at "Diagnostics"
DOI: 10.3390/diagnostics11061084
Abstract: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) triggers disease with nonspecific symptoms that overlap those of infections caused by other seasonal respiratory viruses (RVs), such as the influenza virus (Flu) or respiratory syncytial virus (RSV).… read more here.
Keywords: detection; detection sars; respiratory viruses; assay system ... See more keywords