Clinical evaluation of multiplex RT-PCR assays for the detection of influenza A/B and respiratory syncytial virus using a high throughput system.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of virological methods"
DOI: 10.1016/j.jviromet.2019.03.015
Abstract: BACKGROUND Lower respiratory tract infections are a major threat to public health systems worldwide, with RSV and influenza being the main agents causing hospitalization. In outbreak situations, high-volume respiratory testing is needed. In this study,… read more here.
Keywords: detection influenza; influenza; assays detection; high throughput ... See more keywords
New colony multiplex PCR assays for the detection and discrimination of vancomycin-resistant enterococcal species.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of microbiological methods"
DOI: 10.1016/j.mimet.2017.12.013
Abstract: New colony multiplex PCR assays for detection of seven types of vancomycin-resistance determinants and eight types of Enterococcus species were developed. For 135 enterococcal isolates examined in this study, these assays showed high sensitivity and… read more here.
Keywords: detection; multiplex pcr; new colony; pcr assays ... See more keywords
A comparison of assays for the detection of Cryptosporidium parvum in the feces of scouring calves
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Veterinary Diagnostic Investigation"
DOI: 10.1177/10406387211062181
Abstract: Cryptosporidium parvum is a zoonotic, protozoan parasite that causes potentially life-threatening diarrhea in the host and can be transmitted via the fecal-oral route. C. parvum can infect cattle and may be detected in their feces… read more here.
Keywords: comparison assays; parvum; cryptosporidium parvum; assays detection ... See more keywords
Two extraction-free reverse transcription loop-mediated isothermal amplification assays for detection of SARS-CoV-2
Sign Up to like & getrecommendations! Published in 2021 at "BMC Infectious Diseases"
DOI: 10.1186/s12879-021-06876-0
Abstract: Current assays for detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) rely on time consuming, costly and laboratory based methods for virus isolation, purification and removing inhibitors. To address this limitation, we propose a… read more here.
Keywords: loop mediated; detection; reverse transcription; sars cov ... See more keywords
Comparison of different NAT assays for the detection of microorganisms belonging to the class Mollicutes
Sign Up to like & getrecommendations! Published in 2017 at "BMC Veterinary Research"
DOI: 10.1186/s12917-017-1116-2
Abstract: BackgroundMollicutes detection can be cumbersome due to their slow growth in vitro. For this reason, the use of DNA based on generic molecular tests represents an alternative for rapid, sensitive and specific detection of these… read more here.
Keywords: belonging class; detection; microorganisms belonging; assays detection ... See more keywords
Evaluation of pre-analytical conditions and comparison of the performance of several digital PCR assays for the detection of major EGFR mutations in circulating DNA from non-small cell lung cancers: the CIRCAN_0 study
Sign Up to like & getrecommendations! Published in 2017 at "Oncotarget"
DOI: 10.18632/oncotarget.21256
Abstract: Non invasive somatic detection assays are suitable for repetitive tumor characterization or for detecting the appearance of somatic resistance during lung cancer. Molecular diagnosis based on circulating free DNA (cfDNA) offers the opportunity to track… read more here.
Keywords: detection; pre analytical; analytical conditions; pcr assays ... See more keywords
Development of Loop-Mediated Isothermal Amplification Rapid Diagnostic Assays for the Detection of Klebsiella pneumoniae and Carbapenemase Genes in Clinical Samples
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Molecular Biosciences"
DOI: 10.3389/fmolb.2021.794961
Abstract: Klebsiella pneumoniae is an important pathogenic bacterium commonly associated with human healthcare and community-acquired infections. In recent years, K. pneumoniae has become a significant threat to global public and veterinary health, because of its high… read more here.
Keywords: lamp assays; pneumoniae; detection; assays detection ... See more keywords
Towards Lateral Flow Quantitative Assays: Detection Approaches
Sign Up to like & getrecommendations! Published in 2019 at "Biosensors"
DOI: 10.3390/bios9030089
Abstract: Point-of-care (POC) or bedside analysis is a global trend in modern diagnostics. Progress in POC testing has largely been provided by advanced manufacturing technology for lateral flow (immunochromatographic) test strips. They are widely used to… read more here.
Keywords: flow quantitative; towards lateral; quantitative assays; lateral flow ... See more keywords
Evaluation of Three Commercial PCR Assays for the Detection of Azole-Resistant Aspergillus fumigatus from Respiratory Samples of Immunocompromised Patients
Sign Up to like & getrecommendations! Published in 2021 at "Journal of Fungi"
DOI: 10.3390/jof7020132
Abstract: This is the first study comparing three commercially available PCR assays for the detection of Aspergillus DNA from respiratory specimen of immunocompromised patients and the presence of cyp51A gene mutations. Bronchoalveolar lavages (BALs, N =… read more here.
Keywords: aspergillus fumigatus; pcr; pcr assays; assays detection ... See more keywords
Optimization and Application of Real-Time qPCR Assays in Detection and Identification of Chlamydiales in Products of Domestic Ruminant Abortion
Sign Up to like & getrecommendations! Published in 2023 at "Pathogens"
DOI: 10.3390/pathogens12020290
Abstract: Domestic ruminant abortions due to infectious agents represent an important cause of economic losses in the agricultural industry. This study aimed to optimise and apply qPCR assays for detection of Chlamydiales in domestic ruminant abortion… read more here.
Keywords: ruminant abortion; domestic ruminant; detection; qpcr assays ... See more keywords
Performance of Commercially Available Rapid Serological Assays for the Detection of SARS-CoV-2 Antibodies
Sign Up to like & getrecommendations! Published in 2020 at "Pathogens"
DOI: 10.3390/pathogens9121067
Abstract: The coronavirus disease 2019 (COVID-19) pandemic, caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), continues to spread globally. Although several rapid commercial serological assays have been developed, little is known about their performance… read more here.
Keywords: available rapid; serological assays; performance; sars cov ... See more keywords