In Vitro Assembly of Nucleosomes for Binding/Remodeling Assays.
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DOI: 10.1007/978-1-4939-6630-1_1
Abstract: In order to study the functions and activities of chromatin remodeling enzymes in vitro, it is necessary to be able to reconstitute nucleosomes on DNA templates. In this chapter, we describe procedures for purification of… read more here.
Keywords: nucleosomes binding; vitro assembly; remodeling assays; biology ... See more keywords
Controlling the supramolecular assembly of nucleosomes asymmetrically modified on H4.
Sign Up to like & getrecommendations! Published in 2017 at "Chemical communications"
DOI: 10.1039/c7cc06180c
Abstract: In stem cells, H4 proteins carrying different modifications coexist within single nucleosomes. For functional studies, we report the synthesis of such asymmetric nucleosomes. Asymmetry is achieved by transiently crosslinking H4 by a traceless, protease-removable tag… read more here.
Keywords: controlling supramolecular; nucleosomes asymmetrically; asymmetrically modified; assembly nucleosomes ... See more keywords