Clone of plipastatin biosynthetic gene cluster by transformation-associated recombination technique and high efficient expression in model organism Bacillus subtilis.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of biotechnology"
DOI: 10.1016/j.jbiotec.2018.10.006
Abstract: Plipastatin, a cyclic lipopeptide, exhibits inhibitory activity against filamentous fungi and plays an important role in the prevention of plant diseases, and post-harvest preservation of fruits and vegetables. However, the application of plipastatin has been… read more here.
Keywords: gene cluster; bacillus subtilis; associated recombination; gene ... See more keywords
Efficient assembly of a large fragment of monkeypox virus genome as a qPCR template using dual-selection based transformation-associated recombination.
Sign Up to like & getrecommendations! Published in 2022 at "Virologica Sinica"
DOI: 10.1016/j.virs.2022.02.009
Abstract: Transformation-associated recombination (TAR) has been widely used to assemble large DNA constructs. One of the significant obstacles hindering assembly efficiency is the presence of error-prone DNA repair pathways in yeast, which results in vector backbone… read more here.
Keywords: fragment monkeypox; transformation associated; selection; tar ... See more keywords