Increased recombinant adeno‐associated virus production by HEK293 cells using small molecule chemical additives
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology Journal"
DOI: 10.1002/biot.202200450
Abstract: Recombinant adeno‐associated virus (rAAV) has established itself as a highly efficacious gene delivery vector with a well characterised safety profile allowing broad clinical application. Recent successes in rAAV‐mediated gene therapy clinical trials will continue to… read more here.
Keywords: small molecule; recombinant adeno; associated virus; adeno associated ... See more keywords
Enhancement of Recombinant Adeno‐Associated Virus Production in HEK293 Cells by Application of Small Molecule Additives and Genetic Regulation Based on Perfusion Technique
Sign Up to like & getrecommendations! Published in 2025 at "Biotechnology Journal"
DOI: 10.1002/biot.70053
Abstract: Adeno‐associated virus (AAV) vectors offer numerous advantages, including low immunogenicity and a high safety profile, but their development and wide application are still hindered by some technical limitations. Recent studies have shown that small molecule… read more here.
Keywords: adeno associated; molecule; molecule additives; associated virus ... See more keywords
Improving Aggregation Control of Recombinant Adeno‐Associated Virus Serotype 2 (rAAV2) With Small Sugars and Ionic Salts
Sign Up to like & getrecommendations! Published in 2025 at "Biotechnology Journal"
DOI: 10.1002/biot.70157
Abstract: Two persistent challenges in adeno‐associated virus (AAV) manufacturing are AAV particle aggregation and the separation of full and empty AAV capsids in ion‐exchange (IEX) chromatography processes, which add to AAV purification, formulation, and quality control… read more here.
Keywords: adeno associated; control; small sugars; associated virus ... See more keywords
Process economics evaluation and optimization of adeno-associated virus downstream processing.
Sign Up to like & getrecommendations! Published in 2023 at "Biotechnology and bioengineering"
DOI: 10.1002/bit.28402
Abstract: Adeno-associated virus (AAV) manufacturing has traditionally focused upon lab-scale techniques to culture and purify vector products, leading to limitations in production capacity. The tool presented in this paper assesses the feasibility of using non-scalable technologies… read more here.
Keywords: economics; process economics; associated virus; adeno associated ... See more keywords
Critical challenges and advances in recombinant adeno-associated virus (rAAV) biomanufacturing.
Sign Up to like & getrecommendations! Published in 2023 at "Biotechnology and bioengineering"
DOI: 10.1002/bit.28412
Abstract: Gene therapy is a promising therapeutic approach for genetic and acquired diseases nowadays. Among DNA delivery vectors, recombinant adeno-associated virus (rAAV) is one of the most effective and safest vectors used in commercial drugs and… read more here.
Keywords: raav; recombinant adeno; associated virus; adeno associated ... See more keywords
Enrichment of adeno-associated virus serotype 5 full capsids by anion exchange chromatography with dual salt elution gradients.
Sign Up to like & getrecommendations! Published in 2023 at "Biotechnology and bioengineering"
DOI: 10.1002/bit.28453
Abstract: Adeno-associated virus-based gene therapies have demonstrated substantial therapeutic benefit for the treatment of genetic disorders. In manufacturing processes, viral capsids are produced with and without the encapsidated gene of interest. Capsids devoid of the gene… read more here.
Keywords: associated virus; adeno associated; full capsids; anion exchange ... See more keywords
Removal of empty capsids from high‐dose adeno‐associated virus 9 gene therapies
Sign Up to like & getrecommendations! Published in 2024 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.28737
Abstract: Recombinant adeno‐associated virus, serotype 9 (rAAV9) has shown promise as a gene therapy vector for muscle and central nervous diseases. High‐dose requirements of these therapies present critical safety considerations and biomanufacturing challenges. Notably, the reduction… read more here.
Keywords: adeno associated; empty capsids; removal empty; high dose ... See more keywords
Harnessing cell aggregates for enhanced adeno‐associated virus manufacturing: Cultivation strategies and scale‐up considerations
Sign Up to like & getrecommendations! Published in 2025 at "Biotechnology Progress"
DOI: 10.1002/btpr.3522
Abstract: The possibility to produce recombinant adeno‐associated virus (rAAV) by adherent HEK293T cells was studied in a stirred tank bioreactor (STR) culture of cell aggregates. A proof‐of‐concept of rAAV production was successfully demonstrated in a process… read more here.
Keywords: adeno associated; culture; cell; cell aggregates ... See more keywords
Age‐stratified adeno‐associated virus serotype 3 neutralizing and total antibody prevalence in hemophilia A patients from India
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Medical Virology"
DOI: 10.1002/jmv.27859
Abstract: Gene therapy using an adeno‐associated virus (AAV) vector offers a new treatment option for individuals with monogenetic disorders. The major bottleneck is the presence of pre‐existing anti‐AAV antibodies, which impacts its use. Even very low… read more here.
Keywords: hemophilia patients; associated virus; adeno associated; age ... See more keywords
Extracellular vesicles: nature's nanoparticles for improving gene transfer with adeno-associated virus vectors.
Sign Up to like & getrecommendations! Published in 2018 at "Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology"
DOI: 10.1002/wnan.1488
Abstract: Gene therapy, the ability to treat a disease at the level of nucleic acid, has journeyed from science fiction, to hard lessons learned from early clinical trials, to improved technologies with efficacy in patients for… read more here.
Keywords: associated virus; aav vectors; adeno associated; gene transfer ... See more keywords
Multimodal Production of Adeno-Associated Virus.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9065-8_6
Abstract: Adeno-associated virus (AAV) is an increasingly popular tool in the research laboratory, and use of this viral vector clinically is occurring at an accelerated pace. Nevertheless, despite its popularity, AAV is a relatively cumbersome virus… read more here.
Keywords: production; associated virus; adeno associated; multimodal production ... See more keywords