Increased recombinant adeno‐associated virus production by HEK293 cells using small molecule chemical additives
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology Journal"
DOI: 10.1002/biot.202200450
Abstract: Recombinant adeno‐associated virus (rAAV) has established itself as a highly efficacious gene delivery vector with a well characterised safety profile allowing broad clinical application. Recent successes in rAAV‐mediated gene therapy clinical trials will continue to… read more here.
Keywords: small molecule; recombinant adeno; associated virus; adeno associated ... See more keywords
Process economics evaluation and optimization of adeno-associated virus downstream processing.
Sign Up to like & getrecommendations! Published in 2023 at "Biotechnology and bioengineering"
DOI: 10.1002/bit.28402
Abstract: Adeno-associated virus (AAV) manufacturing has traditionally focused upon lab-scale techniques to culture and purify vector products, leading to limitations in production capacity. The tool presented in this paper assesses the feasibility of using non-scalable technologies… read more here.
Keywords: economics; process economics; associated virus; adeno associated ... See more keywords
Critical challenges and advances in recombinant adeno-associated virus (rAAV) biomanufacturing.
Sign Up to like & getrecommendations! Published in 2023 at "Biotechnology and bioengineering"
DOI: 10.1002/bit.28412
Abstract: Gene therapy is a promising therapeutic approach for genetic and acquired diseases nowadays. Among DNA delivery vectors, recombinant adeno-associated virus (rAAV) is one of the most effective and safest vectors used in commercial drugs and… read more here.
Keywords: raav; recombinant adeno; associated virus; adeno associated ... See more keywords
Enrichment of adeno-associated virus serotype 5 full capsids by anion exchange chromatography with dual salt elution gradients.
Sign Up to like & getrecommendations! Published in 2023 at "Biotechnology and bioengineering"
DOI: 10.1002/bit.28453
Abstract: Adeno-associated virus-based gene therapies have demonstrated substantial therapeutic benefit for the treatment of genetic disorders. In manufacturing processes, viral capsids are produced with and without the encapsidated gene of interest. Capsids devoid of the gene… read more here.
Keywords: associated virus; adeno associated; full capsids; anion exchange ... See more keywords
Age‐stratified adeno‐associated virus serotype 3 neutralizing and total antibody prevalence in hemophilia A patients from India
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Medical Virology"
DOI: 10.1002/jmv.27859
Abstract: Gene therapy using an adeno‐associated virus (AAV) vector offers a new treatment option for individuals with monogenetic disorders. The major bottleneck is the presence of pre‐existing anti‐AAV antibodies, which impacts its use. Even very low… read more here.
Keywords: hemophilia patients; associated virus; adeno associated; age ... See more keywords
Extracellular vesicles: nature's nanoparticles for improving gene transfer with adeno-associated virus vectors.
Sign Up to like & getrecommendations! Published in 2018 at "Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology"
DOI: 10.1002/wnan.1488
Abstract: Gene therapy, the ability to treat a disease at the level of nucleic acid, has journeyed from science fiction, to hard lessons learned from early clinical trials, to improved technologies with efficacy in patients for… read more here.
Keywords: associated virus; aav vectors; adeno associated; gene transfer ... See more keywords
Multimodal Production of Adeno-Associated Virus.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9065-8_6
Abstract: Adeno-associated virus (AAV) is an increasingly popular tool in the research laboratory, and use of this viral vector clinically is occurring at an accelerated pace. Nevertheless, despite its popularity, AAV is a relatively cumbersome virus… read more here.
Keywords: production; associated virus; adeno associated; multimodal production ... See more keywords
Development of sensitive molecular assays for the detection of grapevine leafroll-associated virus 3 in an insect vector
Sign Up to like & getrecommendations! Published in 2019 at "Archives of Virology"
DOI: 10.1007/s00705-019-04310-0
Abstract: Grapevine leafroll-associated virus 3 (GLRaV-3) is an economically significant virus of grapevines, with secondary spread mediated by several species of mealybug and soft scale insects. To better understand virus-vector interactions, sensitive virus detection in these… read more here.
Keywords: detection; grapevine leafroll; associated virus; virus ... See more keywords
Comparative analysis of a Thai congenital-Zika-syndrome-associated virus with a Thai Zika-fever-associated virus
Sign Up to like & getrecommendations! Published in 2020 at "Archives of Virology"
DOI: 10.1007/s00705-020-04667-7
Abstract: In this study, we compared the characteristics of two strains of Zika virus (ZIKV) isolated in Thailand, one isolated from a febrile patient and one isolated from tissues of a fetus medically terminated due to… read more here.
Keywords: congenital zika; thai; associated virus; fever associated ... See more keywords
Survey of grapevine pathogens in Pakistan
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Plant Pathology"
DOI: 10.1007/s42161-019-00263-0
Abstract: Grapevines from 13 vineyards in Pakistan were surveyed for the prevalence of several pathogens. Using RT-qPCR, 257 samples were tested for 19 viruses, phytoplasmas and Xylella fastidiosa. Prevalent viruses were: grapevine virus A (GVA, 47.8%),… read more here.
Keywords: associated virus; grapevine; glrav; virus ... See more keywords
Frustration and Direct Coupling Analyses to Predict Formation and Function of Adeno-Associated Virus.
Sign Up to like & getrecommendations! Published in 2020 at "Biophysical journal"
DOI: 10.1016/j.bpj.2020.12.018
Abstract: Adeno-associated virus (AAV) is a promising gene therapy vector due to its efficient gene delivery and relatively mild immunogenicity. To improve delivery target specificity, researchers use combinatorial and rational library design strategies to generate novel… read more here.
Keywords: protein; associated virus; adeno associated; function ... See more keywords