Characterization of polymeric substance classes in cereal-based beverages using asymmetrical flow field-flow fractionation with a multi-detection system
Sign Up to like & getrecommendations! Published in 2017 at "Analytical and Bioanalytical Chemistry"
DOI: 10.1007/s00216-017-0512-6
Abstract: Cereal-based beverages contain a complex mixture of various polymeric macromolecules including polysaccharides, peptides, and polyphenols. The molar mass of polymers and their degradation products affect different technological and especially sensory parameters of beverages. Asymmetrical flow… read more here.
Keywords: detection; cereal based; asymmetrical flow; based beverages ... See more keywords
Recovery, overloading, and protein interactions in asymmetrical flow field-flow fractionation
Sign Up to like & getrecommendations! Published in 2019 at "Analytical and Bioanalytical Chemistry"
DOI: 10.1007/s00216-019-01673-w
Abstract: AbstractIn asymmetrical flow field-flow fractionation (AF4), similar to other separation techniques, mass recovery and overloading require special attention in order to obtain quantitative results. We conducted a systematic study with five globular proteins of different… read more here.
Keywords: chemistry; flow fractionation; asymmetrical flow; recovery overloading ... See more keywords
Investigation of lipidomic perturbations in oxidatively stressed subcellular organelles and exosomes by asymmetrical flow field-flow fractionation and nanoflow ultrahigh performance liquid chromatography-tandem mass spectrometry.
Sign Up to like & getrecommendations! Published in 2019 at "Analytica chimica acta"
DOI: 10.1016/j.aca.2019.04.069
Abstract: We investigated the effect of oxidative stress (OS) on lipidomic perturbations in the subcellular fractions and exosomes of human embryonic kidney (HEK) 293 cells using asymmetrical flow field-flow fractionation (AF4) and nanoflow ultrahigh performance liquid chromatography-electrospray… read more here.
Keywords: flow fractionation; flow field; field flow; asymmetrical flow ... See more keywords
Continuous asymmetrical flow field-flow fractionation for the purification of proteins and nanoparticles
Sign Up to like & getrecommendations! Published in 2020 at "Separation and Purification Technology"
DOI: 10.1016/j.seppur.2020.116744
Abstract: Abstract We present a novel continuous two-dimensional asymmetrical flow field-flow fractionation (2D-AF4) system that is able to fractionate a feed solution of nano-sized solutes (e.g., proteins, nanoparticles) according to their size in aqueous solvents. The… read more here.
Keywords: fractionation; flow; flow fractionation; asymmetrical flow ... See more keywords
Offline Coupling of Asymmetrical Flow Field-Flow Fractionation and Capillary Electrophoresis for Separation of Extracellular Vesicles.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c03550
Abstract: Extracellular vesicles (EVs) play important roles in cell-to-cell communications and carry high potential as markers targeted in disease diagnosis, prognosis, and therapeutic development. The main obstacles to EV study are their high heterogeneity; low amounts… read more here.
Keywords: flow field; offline coupling; extracellular vesicles; coupling asymmetrical ... See more keywords
Characterizing Non-covalent Protein Complexes Using Asymmetrical Flow Field-Flow Fractionation On-Line Coupled to Native Mass Spectrometry
Sign Up to like & getrecommendations! Published in 2023 at "Analytical Chemistry"
DOI: 10.1021/acs.analchem.2c05049
Abstract: We report an online analytical platform based on the coupling of asymmetrical flow field-flow fractionation (AF4) and native mass spectrometry (nMS) in parallel with UV-absorbance, multi-angle light scattering (MALS), and differential-refractive-index (UV–MALS–dRI) detectors to elucidate… read more here.
Keywords: flow field; field flow; native mass; mass spectrometry ... See more keywords
Pitfalls in Size Characterization of Soft Particles by Dynamic Light Scattering Online Coupled to Asymmetrical Flow Field-Flow Fractionation.
Sign Up to like & getrecommendations! Published in 2017 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.7b03251
Abstract: An asymmetrical flow field-flow fractionation (AF4) technique coupled to a multiangle light scattering (MALS) detector with an embedded dynamic light scattering (DLS) module was introduced to study the size characteristics and shape of soft particles… read more here.
Keywords: field flow; asymmetrical flow; size; flow field ... See more keywords
Ultrafiltration and size exclusion chromatography combined with asymmetrical-flow field-flow fractionation for the isolation and characterisation of extracellular vesicles from urine
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Extracellular Vesicles"
DOI: 10.1080/20013078.2018.1490143
Abstract: ABSTRACT Extracellular vesicles (EVs) have a great potential in clinical applications. However, their isolation from different bodily fluids and their characterisation are currently not optimal or standardised. Here, we report the results of examining the… read more here.
Keywords: extracellular vesicles; characterisation; exclusion chromatography; asymmetrical flow ... See more keywords
Asymmetrical Flow Field-Flow Fractionation Methods for Quantitative Determination and Size Characterization of Thiols and for Mercury Size Speciation Analysis in Organic Matter-Rich Natural Waters
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Chemistry"
DOI: 10.3389/fchem.2022.800696
Abstract: Asymmetrical flow field-flow fractionation (AF4) efficiently separates various macromolecules and nano-components of natural waters according to their hydrodynamic sizes. The online coupling of AF4 with fluorescence (Fluo) and UV absorbance (UV) detectors (FluoD and UVD,… read more here.
Keywords: size; field flow; dom; flow fractionation ... See more keywords
Native RNA Purification Method for Small RNA Molecules Based on Asymmetrical Flow Field-Flow Fractionation
Sign Up to like & getrecommendations! Published in 2022 at "Pharmaceuticals"
DOI: 10.3390/ph15020261
Abstract: RNA molecules provide promising new possibilities for the prevention and treatment of viral infections and diseases. The rapid development of RNA biology and medicine requires advanced methods for the purification of RNA molecules, which allow… read more here.
Keywords: flow field; purification; rna molecules; rna ... See more keywords