Elucidating the Uncoupling of ATP Hydrolysis and Ca2+ Transport in SERCA by Sarcolipin
Sign Up to like & getrecommendations! Published in 2017 at "Biophysical Journal"
DOI: 10.1016/j.bpj.2016.11.2708
Abstract: The activity of the sarco(endo)plasmic reticulum Ca2+ -ATPase (SERCA), which plays a key role in muscle contraction and relaxation, is regulated by sarcolipin (SLN), a 31-residue transmembrane peptide. Sarcolipin has been shown to uncouple ATP… read more here.
Keywords: atp hydrolysis; serca; hydrolysis ca2; ca2 transport ... See more keywords
Insights into the Cooperative Nature of ATP Hydrolysis in Actin Filaments.
Sign Up to like & getrecommendations! Published in 2018 at "Biophysical journal"
DOI: 10.1016/j.bpj.2018.08.034
Abstract: Actin filaments continually assemble and disassemble within a cell. Assembled filaments "age" as a bound nucleotide ATP within each actin subunit quickly hydrolyzes followed by a slower release of the phosphate Pi, leaving behind a… read more here.
Keywords: atp hydrolysis; cooperative nature; hydrolysis; actin filaments ... See more keywords
Yeast Chd1p Unwraps the Exit Side DNA upon ATP Binding to Facilitate the Nucleosome Translocation Occurring upon ATP Hydrolysis.
Sign Up to like & getrecommendations! Published in 2020 at "Biochemistry"
DOI: 10.1021/acs.biochem.0c00747
Abstract: Chromodomain-helicase-DNA-binding protein 1 (CHD1) remodels chromatin by translocating nucleosomes along DNA, but its mechanism remains poorly understood. We use single-molecule fluorescence experiments to clarify the mechanism by which yeast CHD1 (Chd1p) remodels nucleosomes. We find… read more here.
Keywords: atp hydrolysis; nucleosome translocation; upon atp; dna ... See more keywords
Role of the Conserved DECH-Box Cysteine in Coupling Hepatitis C Virus Helicase-Catalyzed ATP Hydrolysis to RNA Unwinding.
Sign Up to like & getrecommendations! Published in 2018 at "Biochemistry"
DOI: 10.1021/acs.biochem.8b00796
Abstract: DECH-box proteins are a subset of DExH/D-box superfamily 2 helicases possessing a conserved Asp-Glu-Cys-His motif in their ATP binding site. The conserved His helps position the Asp and Glu residues, which coordinate the divalent metal… read more here.
Keywords: cys; atp hydrolysis; role; helicase ... See more keywords
Molecular Basis of RNA-Driven ATP Hydrolysis in DExH-Box Helicases.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.2c11980
Abstract: The spliceosome machinery catalyzes precursor messenger (pre-m)RNA splicing. In each cycle, the spliceosome experiences massive compositional and conformational remodeling fueled by the concerted action of specific RNA-dependent ATPases/helicases. Intriguingly, these enzymes are allosterically activated to… read more here.
Keywords: dexh box; rna; atp hydrolysis;
Spectroscopic glimpses of the transition state of ATP hydrolysis trapped in a bacterial DnaB helicase
Sign Up to like & getrecommendations! Published in 2021 at "Nature Communications"
DOI: 10.1038/s41467-021-25599-z
Abstract: The ATP hydrolysis transition state of motor proteins is a weakly populated protein state that can be stabilized and investigated by replacing ATP with chemical mimics. We present atomic-level structural and dynamic insights on a… read more here.
Keywords: state; bacterial dnab; atp hydrolysis; transition state ... See more keywords
ATP hydrolysis and nucleotide exit enhance maltose translocation in the MalFGK2E importer
Sign Up to like & getrecommendations! Published in 2021 at "Scientific Reports"
DOI: 10.1038/s41598-021-89556-y
Abstract: ATP binding cassette (ABC) transporters employ ATP hydrolysis to harness substrate translocation across membranes. The Escherichia coli MalFGK2E maltose importer is an example of a type I ABC importer and a model system for this… read more here.
Keywords: hydrolysis nucleotide; importer; atp hydrolysis; hydrolysis ... See more keywords
Hierarchical dynamics in allostery following ATP hydrolysis monitored by single molecule FRET measurements and MD simulations†
Sign Up to like & getrecommendations! Published in 2021 at "Chemical Science"
DOI: 10.1039/d0sc06134d
Abstract: We report on a study that combines advanced fluorescence methods with molecular dynamics (MD) simulations to cover timescales from nanoseconds to milliseconds for a large protein. This allows us to delineate how ATP hydrolysis in… read more here.
Keywords: protein; hierarchical dynamics; atp hydrolysis; allostery following ... See more keywords
Differential regulation of ATP hydrolysis of RIG-I-like receptors by transactivation response RNA-binding protein
Sign Up to like & getrecommendations! Published in 2023 at "Bioscience Reports"
DOI: 10.1042/bsr20222152
Abstract: Abstract Retinoic acid inducible gene (RIG)-I-like receptors (RLRs), including RIG-I, melanoma differentiation associated-5 (MDA5), and laboratory of genetics and physiology 2 (LGP2), play pivotal roles in viral RNA sensing to initiate antiviral interferon (IFN) responses.… read more here.
Keywords: rna; trbp; atp hydrolysis; response ... See more keywords
Road-blocker HSP disease mutation disrupts pre-organization for ATP hydrolysis in kinesin through a second sphere control
Sign Up to like & getrecommendations! Published in 2022 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2215170120
Abstract: Significance Kinesin, an amazing biological machine, converts chemical energy from the ATP hydrolysis into mechanical energy to perform different cellular activities. Several single-point gene-level mutations in the neuronal kinesin motor (KIF5A) are linked to a… read more here.
Keywords: pre organization; kinesin second; second sphere; atp hydrolysis ... See more keywords
Pentapeptide repeat protein QnrB1 requires ATP hydrolysis to rejuvenate poisoned gyrase complexes
Sign Up to like & getrecommendations! Published in 2021 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkaa1266
Abstract: Abstract DNA gyrase, a type II topoisomerase found predominantly in bacteria, is the target for a variety of ‘poisons’, namely natural product toxins (e.g. albicidin, microcin B17) and clinically important synthetic molecules (e.g. fluoroquinolones). Resistance… read more here.
Keywords: pentapeptide repeat; atp hydrolysis; gyrase; requires atp ... See more keywords