A Conserved Histidine Residue Drives Extein Dependence in an Enhanced Atypically Split Intein.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.2c08985
Abstract: Split intein-mediated protein trans-splicing (PTS) is widely applied in chemical biology and biotechnology to carry out traceless and specific protein ligation. However, the external residues immediately flanking the intein (exteins) can reduce the splicing rate,… read more here.
Keywords: dependence; dependence enhanced; atypically split; extein dependence ... See more keywords
Structural and biochemical analysis of a novel atypically split intein reveals a conserved histidine specific to cysteine-less inteins
Sign Up to like & getrecommendations! Published in 2023 at "Chemical Science"
DOI: 10.1039/d3sc01200j
Abstract: Protein trans-splicing mediated by a split intein reconstitutes a protein backbone from two parts. This virtually traceless autoprocessive reaction provides the basis for numerous protein engineering applications. Protein splicing typically proceeds through two thioester or… read more here.
Keywords: atypically split; histidine; cysteine less; split intein ... See more keywords