Preparation of Electrocompetent Staphylococcus aureus Cells and Plasmid Transformation.
Sign Up to like & getrecommendations! Published in 2023 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot107947
Abstract: This protocol is part of a series of methodologies for the construction of an in-frame gene deletion in Staphylococcus aureus strain RN4220. Having previously described how an allelic-exchange plasmid containing a desired gene deletion (in… read more here.
Keywords: aureus cells; plasmid; staphylococcus aureus; aureus ... See more keywords
An improved assay for rapid detection of viable Staphylococcus aureus cells by incorporating surfactant and PMA treatments in qPCR
Sign Up to like & getrecommendations! Published in 2018 at "BMC Microbiology"
DOI: 10.1186/s12866-018-1273-x
Abstract: BackgroundStaphylococcus aureus is an important human pathogen causing a variety of life-threatening diseases. Rapid and accurate detection of Staphylococcus aureus is a necessity for prevention of outbreaks caused by this pathogen. PCR is a useful… read more here.
Keywords: pma; detection; staphylococcus aureus; detection viable ... See more keywords