Integrating dynamic clamping with automated patch clamping devices: Adding virtual IK1 channels to iPSC-CM
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Pharmacological and Toxicological Methods"
DOI: 10.1016/j.vascn.2018.01.472
Abstract: An important aspect of the CiPA (Comprehensive In Vitro Proarrhythmia Assay) proposal is the use of human stem cell-derived cardiomyocytes and the confirmation of their predictivity in drug safety assays. The benefits of this cell… read more here.
Keywords: integrating dynamic; clamping; clamping automated; dynamic clamping ... See more keywords
Reliable identification of cardiac liability in drug discovery using automated patch clamp: Benchmarking best practices and calibration standards for improved proarrhythmic assessment.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of pharmacological and toxicological methods"
DOI: 10.1016/j.vascn.2020.106884
Abstract: INTRODUCTION Screening compounds for activity on the hERG channel using patch clamp is a crucial part of safety testing. Automated patch clamp (APC) is becoming widely accepted as an alternative to manual patch clamp in… read more here.
Keywords: patch clamp; patch; automated patch; reliable identification ... See more keywords
Unattended screening workflow in a 384-well automated patch clamp system
Sign Up to like & getrecommendations! Published in 2021 at "Journal of Pharmacological and Toxicological Methods"
DOI: 10.1016/j.vascn.2021.106970
Abstract: Within ion channel working group efforts (Fermini et al., 2015), reference compounds were tested across multiple sites and automated patch clamp (APC) platforms (Kramer et al., 2020). Resulting IC50 values were variable between drugs and… read more here.
Keywords: patch clamp; unattended screening; screening workflow; automated patch ... See more keywords
Reliable identification of cardiac conduction abnormalities in drug discovery using automated patch clamp II: Best practices for Nav1.5 peak current in a high throughput screening environment.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of pharmacological and toxicological methods"
DOI: 10.1016/j.vascn.2021.107125
Abstract: INTRODUCTION For reliable identification of cardiac safety risk, compounds should be screened for activity on cardiac ion channels in addition to hERG, including NaV1.5 and CaV1.2. We identified different parameters that might affect IC50s of… read more here.
Keywords: identification cardiac; patch clamp; automated patch; nav1 peak ... See more keywords
Biophysical characterization of light-gated ion channels using planar automated patch clamp
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Molecular Neuroscience"
DOI: 10.3389/fnmol.2022.976910
Abstract: Channelrhodopsins (ChRs) are proteins that guide phototaxis in protists and exhibit light-gated channel conductance when their genes are heterologously expressed in mammalian cells. ChRs are widely used as molecular tools to control neurons and cardiomyocytes… read more here.
Keywords: automated patch; patch clamp; light gated; patch ... See more keywords
There is no F in APC: Using physiological fluoride-free solutions for high throughput automated patch clamp experiments
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Molecular Neuroscience"
DOI: 10.3389/fnmol.2022.982316
Abstract: Fluoride has been used in the internal recording solution for manual and automated patch clamp experiments for decades because it helps to improve the seal resistance and promotes longer lasting recordings. In manual patch clamp,… read more here.
Keywords: clamp experiments; fluoride free; automated patch; patch clamp ... See more keywords
Automated Patch-Clamp and Induced Pluripotent Stem Cell-Derived Cardiomyocytes: A Synergistic Approach in the Study of Brugada Syndrome
Sign Up to like & getrecommendations! Published in 2023 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms24076687
Abstract: The development of high-throughput automated patch-clamp technology is a recent breakthrough in the field of Brugada syndrome research. Brugada syndrome is a heart disorder marked by abnormal electrocardiographic readings and an elevated risk of sudden… read more here.
Keywords: patch clamp; brugada syndrome; derived cardiomyocytes; automated patch ... See more keywords
Nebivolol as a Potent TRPM8 Channel Blocker: A Drug-Screening Approach through Automated Patch Clamping and Ligand-Based Virtual Screening
Sign Up to like & getrecommendations! Published in 2022 at "Membranes"
DOI: 10.3390/membranes12100954
Abstract: Transient Receptor Potential Melastatin 8 (TRPM8) from the melastatin TRP channel subfamily is a non-selective Ca2+-permeable ion channel with multimodal gating which can be activated by low temperatures and cooling compounds, such as menthol and… read more here.
Keywords: virtual screening; ligand based; trpm8; automated patch ... See more keywords