Revisiting the unique structure of autonomously replicating sequences in Yarrowia lipolytica and its role in pathway engineering.
Sign Up to like & getrecommendations! Published in 2021 at "Applied microbiology and biotechnology"
DOI: 10.1007/s00253-021-11399-4
Abstract: Production of industrially relevant compounds in microbial cell factories can employ either genomes or plasmids as an expression platform. Selection of plasmids as pathway carriers is advantageous for rapid demonstration but poses a challenge of… read more here.
Keywords: autonomously replicating; replicating sequences; unique structure; role ... See more keywords
Frequency and patterns of ribonucleotide incorporation around autonomously replicating sequences in yeast reveal the division of labor of replicative DNA polymerases
Sign Up to like & getrecommendations! Published in 2021 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkab801
Abstract: Abstract Ribonucleoside triphosphate (rNTP) incorporation in DNA by DNA polymerases is a frequent phenomenon that results in DNA structural change and genome instability. However, it is unclear whether the rNTP incorporation into DNA follows any… read more here.
Keywords: autonomously replicating; dna; rntp incorporation; dna polymerases ... See more keywords
A Stable, Autonomously Replicating Plasmid Vector Containing Pichia pastoris Centromeric DNA
Sign Up to like & getrecommendations! Published in 2018 at "Applied and Environmental Microbiology"
DOI: 10.1128/aem.02882-17
Abstract: Secretory production of recombinant proteins is the most important application of the methylotrophic yeast Pichia pastoris, a species that permits mass production of heterologous proteins. To date, the genetic engineering of P. pastoris has relied… read more here.
Keywords: plasmid vector; autonomously replicating; centromeric dna; pastoris ... See more keywords
Autonomously Replicating RNAs of Bungowannah Pestivirus: ERNS Is Not Essential for the Generation of Infectious Particles
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DOI: 10.1128/jvi.00436-20
Abstract: The proteins NPRO and ERNS are unique for the genus Pestivirus, but only NPRO has been demonstrated to be nonessential for in vitro growth. While this was also speculated for ERNS, it has always been… read more here.
Keywords: bupv erns; generation infectious; bupv; autonomously replicating ... See more keywords
A Gemini Virus-Derived Autonomously Replicating System for HDR-Mediated Genome Editing of the EPSP Synthase Gene in Indica Rice
Sign Up to like & getrecommendations! Published in 2025 at "Plants"
DOI: 10.3390/plants14030477
Abstract: CRISPR/Cas9-mediated homology-directed repair (HDR) is a powerful tool for precise genome editing in plants, but its efficiency remains low, particularly for targeted amino acid substitutions or gene knock-ins. Successful HDR requires the simultaneous presence of… read more here.
Keywords: system; autonomously replicating; virus; genome editing ... See more keywords