A homozygous hypomorphic BNIP1 variant causes an increase in autophagosomes and reduced autophagic flux and results in a spondylo‐epiphyseal dysplasia
Sign Up to like & getrecommendations! Published in 2022 at "Human Mutation"
DOI: 10.1002/humu.24368
Abstract: BNIP1 (BCL2 interacting protein 1) is a soluble N‐ethylmaleimide‐sensitive factor‐attachment protein receptor involved in ER membrane fusion. We identified the homozygous BNIP1 intronic variant c.84+3A>T in the apparently unrelated patients 1 and 2 with disproportionate… read more here.
Keywords: bnip1; epiphyseal dysplasia; increase; spondylo epiphyseal ... See more keywords
Inhibition of autophagic flux by the curcumin analog EF‐24 and its antiproliferative effect on MCF‐7 cancer cells
Sign Up to like & getrecommendations! Published in 2023 at "Journal of Biochemical and Molecular Toxicology"
DOI: 10.1002/jbt.23307
Abstract: 5‐Bis[(2‐fluorophenyl)methylene]‐4‐piperidinone (EF‐24) is a curcumin analog, which was identified for its physiochemical stability and diverse pharmacological functions. In the present study, EF‐24 was added to the breast cancer cell line MCF‐7 and its cellular effects… read more here.
Keywords: mcf; autophagic flux; curcumin analog; mcf cells ... See more keywords
Autophagic flux inhibition, apoptosis, and mitochondrial dysfunction in bile acids‐induced impairment of human placental trophoblast
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Cellular Physiology"
DOI: 10.1002/jcp.30774
Abstract: Intrahepatic cholestasis of pregnancy (ICP) is a common pregnancy‐specific disease, characterized by increased bile acid levels and adverse fetal outcomes. We previously reported excessive bile acids led to dysfunction of placental trophoblasts in ICP. However,… read more here.
Keywords: autophagic flux; mitochondrial dysfunction; bile acids; dysfunction ... See more keywords
Blunting TRPML1 channels protects myocardial ischemia/reperfusion injury by restoring impaired cardiomyocyte autophagy
Sign Up to like & getrecommendations! Published in 2022 at "Basic Research in Cardiology"
DOI: 10.1007/s00395-022-00930-x
Abstract: Accumulating evidence suggests that autophagy dysfunction plays a critical role in myocardial ischemia/reperfusion (I/R) injury. However, the underling mechanism of malfunctional autophagy in the cardiomyocytes subjected to I/R has not been well defined. As a… read more here.
Keywords: injury; autophagic flux; trpml1; ischemia reperfusion ... See more keywords
Autophagic flux is essential for the downregulation of D-dopachrome tautomerase by atractylenolide I to ameliorate intestinal adenoma formation
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Cell Communication and Signaling"
DOI: 10.1007/s12079-018-0454-6
Abstract: Colorectal cancer is generally believed to progress through an adenoma - carcinoma sequence. Adenomatous polyposis coli (APC) mutations serve as the initiating event in adenoma formation. The ApcMin/+ mouse harbors a mutation in the APC… read more here.
Keywords: adenoma formation; adenoma; intestinal adenoma; formation ... See more keywords
Autophagic flux assessment by immunoblot.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in cell biology"
DOI: 10.1016/bs.mcb.2020.10.005
Abstract: Autophagy is one of the main adaptive mechanisms to maintain cellular homeostasis in response to multiple stresses. During autophagy diverse cellular components such as damaged organelles or superfluous proteins are targeted for lysosomal degradation. Importantly,… read more here.
Keywords: autophagic flux; immunoblot; biology; assessment immunoblot ... See more keywords
Assessment of EGFP-Q74 degradation for the measurement of autophagic flux.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in cell biology"
DOI: 10.1016/bs.mcb.2020.10.018
Abstract: Autophagy plays a major role in physiological and pathological processes. The quantitation of the abundance of autophagy-specific substrates constitutes an efficient strategy for assessing autophagic activity. Here, we provide a detailed protocol for quantifying the… read more here.
Keywords: autophagic flux; egfp q74; q74 degradation; assessment egfp ... See more keywords
Multiplexed quantification of autophagic flux by imaging flow cytometry.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in cell biology"
DOI: 10.1016/bs.mcb.2021.02.005
Abstract: Imaging flow cytometry allows for the quantitative assessment of fluorescent signals at the subcellular level. Here, we describe the use of a biosensor cell line, namely, U2OS osteosarcoma cells equipped with a fusion protein comprising… read more here.
Keywords: flux imaging; autophagic flux; fluorescent; imaging flow ... See more keywords
Graphene oxide induces p62/SQSTM-dependent apoptosis through the impairment of autophagic flux and lysosomal dysfunction in PC12 cells.
Sign Up to like & getrecommendations! Published in 2018 at "Acta biomaterialia"
DOI: 10.1016/j.actbio.2018.09.057
Abstract: Graphene oxide (GO), as a two-dimensional carbon nanosheet, has been extensively studied for potential biomedical applications due to its notable properties. Although a growing number of studies have investigated the adverse effects of GO nanosheets,… read more here.
Keywords: graphene oxide; autophagic flux; pc12 cells; apoptosis ... See more keywords
Impaired autophagic flux contributes to muscle atrophy in obesity by affecting muscle degradation and regeneration.
Sign Up to like & getrecommendations! Published in 2020 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2020.02.110
Abstract: Increased proteolytic activity has been widely associated with skeletal muscle atrophy. However, elevated proteolysis is also critical for the maintenance of intracellular homeostasis. In this study, we aimed to investigate the significance of autophagy in… read more here.
Keywords: affecting muscle; muscle; muscle degradation; autophagic flux ... See more keywords
Modulating autophagic flux via ROS-responsive targeted micelles to restore neuronal proteostasis in Alzheimer's disease
Sign Up to like & getrecommendations! Published in 2021 at "Bioactive Materials"
DOI: 10.1016/j.bioactmat.2021.09.017
Abstract: Abstract Compromised autophagy and defective lysosomal clearance significantly contribute to impaired neuronal proteostasis, which represents a hallmark of Alzheimer's disease (AD) and other age-related neurodegenerative disorders. Growing evidence has implicated that modulating autophagic flux, instead… read more here.
Keywords: modulating autophagic; alzheimer disease; autophagic flux; neuronal proteostasis ... See more keywords