Quantitative characterization of the auxin-inducible degron: a guide for dynamic protein depletion in single yeast cells
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Reports"
DOI: 10.1038/s41598-017-04791-6
Abstract: Perturbations are essential for the interrogation of biological systems. The auxin-inducible degron harbors great potential for dynamic protein depletion in yeast. Here, we thoroughly and quantitatively characterize the auxin-inducible degron in single yeast cells. We… read more here.
Keywords: protein; auxin inducible; inducible degron; yeast ... See more keywords
An expanded auxin-inducible degron toolkit for Caenorhabditis elegans
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DOI: 10.1093/genetics/iyab006
Abstract: Abstract The auxin-inducible degron (AID) system has emerged as a powerful tool to conditionally deplete proteins in a range of organisms and cell types. Here, we describe a toolkit to augment the use of the… read more here.
Keywords: repair templates; auxin inducible; caenorhabditis elegans; inducible degron ... See more keywords
The auxin-inducible degron 2 (AID2) system enables controlled protein knockdown during embryogenesis and development in Caenorhabditis elegans.
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DOI: 10.1093/genetics/iyab218
Abstract: Targeted protein degradation using the auxin-inducible degron (AID) system is garnering attention in the research field of Caenorhabditis elegans, because of the rapid and efficient target depletion it affords, which can be controlled by treating… read more here.
Keywords: system; caenorhabditis elegans; degradation; auxin inducible ... See more keywords
Auxin-inducible degron system: an efficient protein degradation tool to study protein function.
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DOI: 10.2144/btn-2022-0108
Abstract: Targeted protein degradation, with its rapid protein depletion kinetics, allows the measurement of acute changes in the cell. The auxin-inducible degron (AID) system, rapidly degrades AID-tagged proteins only in the presence of auxin. The AID system… read more here.
Keywords: system; auxin inducible; protein degradation; aid ... See more keywords
A One-step strategy to target essential factors with auxin-inducible degron system in mouse embryonic stem cells
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DOI: 10.3389/fcell.2022.964119
Abstract: The self-renewal and pluripotency of embryonic stem cells (ESCs) are conferred by networks including transcription factors and histone modifiers. The Auxin-inducible degron (AID) system can rapidly and reversibly degrade its target proteins and is becoming… read more here.
Keywords: system; auxin inducible; inducible degron; embryonic stem ... See more keywords
Rapid and specific degradation of endogenous proteins in mouse models using auxin-inducible degrons
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DOI: 10.7554/elife.77987
Abstract: Auxin-inducible degrons are a chemical genetic tool for targeted protein degradation and are widely used to study protein function in cultured mammalian cells. Here, we develop CRISPR-engineered mouse lines that enable rapid and highly specific… read more here.
Keywords: auxin inducible; inducible degrons; proteins mouse; endogenous proteins ... See more keywords