PV interneurons evoke astrocytic Ca2+ responses in awake mice, which contributes to neurovascular coupling.
Sign Up to like & getrecommendations! Published in 2023 at "Glia"
DOI: 10.1002/glia.24370
Abstract: Neurovascular coupling (NVC) modulates cerebral blood flow to match increased metabolic demand during neuronal excitation. Activation of inhibitory interneurons also increase blood flow, but the basis for NVC caused by interneurons is unclear. While astrocyte… read more here.
Keywords: neurovascular coupling; astrocytic ca2; awake mice; ca2 responses ... See more keywords
Functional imaging of visual cortical layers and subplate in awake mice with optimized three-photon microscopy
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DOI: 10.1038/s41467-018-08179-6
Abstract: Two-photon microscopy is used to image neuronal activity, but has severe limitations for studying deeper cortical layers. Here, we developed a custom three-photon microscope optimized to image a vertical column of the cerebral cortex > 1 mm in… read more here.
Keywords: photon; awake mice; microscopy; cortical layers ... See more keywords
Kilohertz two-photon brain imaging in awake mice
Sign Up to like & getrecommendations! Published in 2019 at "Nature Methods"
DOI: 10.1038/s41592-019-0597-2
Abstract: Two-photon microscopy is a mainstay technique for imaging in scattering media and normally provides frame-acquisition rates of ~10–30 Hz. To track high-speed phenomena, we created a two-photon microscope with 400 illumination beams that collectively sample 95,000–211,000 µm2… read more here.
Keywords: photon; photon brain; awake mice; two photon ... See more keywords