Backbone resonance assignments of monomeric SOD1 in dilute and crowded environments
Sign Up to like & getrecommendations! Published in 2017 at "Biomolecular NMR Assignments"
DOI: 10.1007/s12104-016-9724-5
Abstract: Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease that leads to movement disorders. In motor neurons of ALS patients, intracellular aggregates of superoxide dismutase 1 (SOD1) have often been observed. To elucidate the aggregation… read more here.
Keywords: protein; backbone resonance; sod1; monomeric sod1 ... See more keywords
Backbone resonance assignments for the SET domain of human methyltransferase NSD3 in complex with its cofactor
Sign Up to like & getrecommendations! Published in 2017 at "Biomolecular NMR Assignments"
DOI: 10.1007/s12104-017-9753-8
Abstract: NSD3 is a histone H3 methyltransferase that plays an important role in chromatin biology. A construct containing the methyltransferase domain encompassing residues Q1049-K1299 of human NSD3 was obtained and biochemical activity was demonstrated using histone… read more here.
Keywords: backbone resonance; resonance assignments; methyltransferase; nsd3 ... See more keywords
1H, 15N, 13C backbone resonance assignment of the C-terminal domain of enzyme I from Thermoanaerobacter tengcongensis
Sign Up to like & getrecommendations! Published in 2018 at "Biomolecular NMR Assignments"
DOI: 10.1007/s12104-017-9788-x
Abstract: Phosphoenolpyruvate binding to the C-terminal domain (EIC) of enzyme I of the bacterial phosphotransferase system (PTS) initiates a phosphorylation cascade that results in sugar translocation across the cell membrane and controls a large number of… read more here.
Keywords: backbone resonance; terminal domain; thermoanaerobacter tengcongensis; 15n 13c ... See more keywords
1H, 15N, 13C backbone resonance assignment of human Alkbh5
Sign Up to like & getrecommendations! Published in 2018 at "Biomolecular NMR Assignments"
DOI: 10.1007/s12104-018-9826-3
Abstract: N6-methyladenosine (m6A) is the most abundant and reversible post-transcriptional modification in eukaryotic mRNA and long non-coding RNA (lncRNA). The central role of m6A in various physiological processes has generated considerable biological and pharmacological interest. Alkbh5… read more here.
Keywords: backbone resonance; human alkbh5; assignment; 15n 13c ... See more keywords
Backbone resonance assignment for the N-terminal region of bacterial tRNA-(N1G37) methyltransferase
Sign Up to like & getrecommendations! Published in 2019 at "Biomolecular NMR Assignments"
DOI: 10.1007/s12104-018-9849-9
Abstract: Bacterial tRNA (guanine37-N1)-methyltransferase (TrmD) is an important antibacterial target due to its essential role in translation. TrmD has two domains connected with a flexible linker. The N-terminal domain (NTD) of TrmD contains the S-adenosyl-l-methionine (SAM)… read more here.
Keywords: backbone resonance; methyltransferase; trna; resonance assignment ... See more keywords
Backbone resonance assignments and secondary structure of Ebola nucleoprotein 600–739 construct
Sign Up to like & getrecommendations! Published in 2019 at "Biomolecular NMR Assignments"
DOI: 10.1007/s12104-019-09898-7
Abstract: Ebola viral infections have resulted in several deadly epidemics in recent years in West and Central Africa. Because only one of the seven proteins encoded by the viral genome possesses enzymatic activity, disruption of protein–protein… read more here.
Keywords: backbone resonance; resonance assignments; secondary structure; 600 739 ... See more keywords
1H, 15N and 13C backbone resonance assignments of the P146A variant of β-phosphoglucomutase from Lactococcus lactis in its substrate-free form
Sign Up to like & getrecommendations! Published in 2019 at "Biomolecular Nmr Assignments"
DOI: 10.1007/s12104-019-09904-y
Abstract: β-Phosphoglucomutase (βPGM) is a magnesium-dependent phosphoryl transfer enzyme that catalyses the reversible isomerisation of β-glucose 1-phosphate and glucose 6-phosphate, via two phosphoryl transfer steps and a β-glucose 1,6-bisphosphate intermediate. Substrate-free βPGM is an essential component… read more here.
Keywords: backbone resonance; substrate free; 15n 13c; backbone ... See more keywords
Backbone resonance assignment of the cAMP-binding domains of the protein kinase A regulatory subunit Iα.
Sign Up to like & getrecommendations! Published in 2021 at "Biomolecular NMR assignments"
DOI: 10.1007/s12104-021-10033-8
Abstract: Protein kinase A (PKA) is the main receptor for the universal cAMP second messenger. PKA is a tetramer with two catalytic (C) and two regulatory (R) subunits, each including two tandem cAMP-binding domains, i.e. CBD-A… read more here.
Keywords: backbone resonance; camp; binding domains; camp binding ... See more keywords
Backbone resonance assignment of PDI b'xa' domain construct.
Sign Up to like & getrecommendations! Published in 2021 at "Biomolecular NMR assignments"
DOI: 10.1007/s12104-021-10038-3
Abstract: Human protein disulfide isomerase (PDI), a protein containing 4 domains a, b, b', a', disordered x linker and C-terminus, plays critical roles in disulfide bond reactions and proper protein folding in the endoplasmic reticulum. The… read more here.
Keywords: backbone resonance; construct; domain; assignment pdi ... See more keywords
Co-refolding of a functional complex of Dengue NS3 protease and NS2B co-factor domain and backbone resonance assignment by solution NMR.
Sign Up to like & getrecommendations! Published in 2017 at "Protein expression and purification"
DOI: 10.1016/j.pep.2017.07.002
Abstract: A novel approach for separate expression of dengue virus NS3 protease and its NS2B cofactor domain is described in this paper. The two proteins are expressed in E.coli and purified separately and subsequently efficiently co-refolded… read more here.
Keywords: backbone resonance; protease ns2b; ns3 protease; resonance ... See more keywords