One-Step Bacterial Artificial Chromosome (BAC) Modification: Preparation of Plasmids.
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DOI: 10.1101/pdb.prot098095
Abstract: In the one-step approach to bacterial artificial chromosome (BAC) modification, two plasmids are introduced into the BAC host cells. The shuttle pLD53.SC2, carrying the EFGP reporter sequence and requiring the π protein to replicate, must… read more here.
Keywords: bac; bac modification; one step; chromosome bac ... See more keywords
One-Step Bacterial Artificial Chromosome (BAC) Modification: Preparation of the A Homology Arm (A-Box).
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DOI: 10.1101/pdb.prot098103
Abstract: The one-step approach to bacterial artificial chromosome (BAC) modification requires that only one homology arm be cloned into the shuttle vector (in the example presented here, we use the "A-box"). The homology arm, which in… read more here.
Keywords: bac; one step; bacterial artificial; homology arm ... See more keywords
Use of a Bacterial Artificial Chromosome to Generate Recombinant SARS-CoV-2 Expressing Robust Levels of Reporter Genes
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DOI: 10.1128/spectrum.02732-22
Abstract: We have established a bacterial artificial chromosome (BAC)-based RG system to generate recombinant severe acute respiratory syndrome coronavirus 2 (rSARS-CoV-2) and to engineer reporter-expressing recombinant viruses to assess viral infection in vitro and in vivo.… read more here.
Keywords: reporter; generate; bac based; rsars cov ... See more keywords
Combined use of bacterial artificial chromosomes-on-beads with karyotype detection improves prenatal diagnosis
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DOI: 10.1186/s13039-019-0416-6
Abstract: BackgroundThis study evaluated the individual and combined diagnostic performance of the bacterial artificial chromosomes (BACs)-on-Beads (BoBs™) assay and conventional karyotyping for the prenatal detection of chromosomal abnormalities in pregnant women who were 35 or more… read more here.
Keywords: detection; conventional karyotyping; artificial chromosomes; bacterial artificial ... See more keywords
ESI mutagenesis: a one-step method for introducing mutations into bacterial artificial chromosomes
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DOI: 10.26508/lsa.202000836
Abstract: A simple and efficient recombineering-based method for introducing point mutations into bacterial artificial chromosomes using an artificial intron cassette. Bacterial artificial chromosome (BAC)–based transgenes have emerged as a powerful tool for controlled and conditional interrogation… read more here.
Keywords: method introducing; esi mutagenesis; step; bacterial artificial ... See more keywords