Articles with "bacterial cells" as a keyword



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Production of Properly Folded NS1 Protein in Bacterial Cells.

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Published in 2022 at "Methods in molecular biology"

DOI: 10.1007/978-1-0716-1879-0_3

Abstract: Dengue virus (DENV) NS1 protein is a multifunctional protein involved in several pathogenic processes but also has been described as a protective antigen suitable for eliciting humoral response against DENV. NS1 is essential for virus… read more here.

Keywords: protein; bacterial cells; production properly; properly folded ... See more keywords
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Quantitative Super-Resolution Imaging of Small RNAs in Bacterial Cells.

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Published in 2018 at "Methods in molecular biology"

DOI: 10.1007/978-1-4939-7634-8_12

Abstract: We present a method for the quantification of small regulatory RNAs (sRNAs) in bacteria, by combining single-molecule fluorescence in situ hybridization (smFISH), super-resolved single-fluorophore microscopy, and clustering analysis. Compared to smFISH imaging with diffraction-limited fluorescence… read more here.

Keywords: bacterial cells; resolution imaging; imaging small; microscopy ... See more keywords
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Light scattering on PHA granules protects bacterial cells against the harmful effects of UV radiation

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Published in 2018 at "Applied Microbiology and Biotechnology"

DOI: 10.1007/s00253-018-8760-8

Abstract: Numerous prokaryotes accumulate polyhydroxyalkanoates (PHA) in the form of intracellular granules. The primary function of PHA is the storage of carbon and energy. Nevertheless, there are numerous reports that the presence of PHA granules in… read more here.

Keywords: scattering pha; bacterial cells; pha; light scattering ... See more keywords
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A Novel ZnONPs/PVA-Functionalized Biomaterials for Bacterial Cells Immobilization and its Strengthening Effects on Quinoline Biodegradation

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Published in 2017 at "Current Microbiology"

DOI: 10.1007/s00284-017-1382-z

Abstract: A novel bacterial cells immobilized carrier (ZnONPs/PVA), polyvinyl alcohol (PVA) composites decorated with ZnO nanoparticles (ZnO NPs), was prepared and used for immobilization of the strain Ochrobactrum sp. LC-1, and subsequently for quinoline degrading in… read more here.

Keywords: bacterial cells; immobilization; pva; quinoline biodegradation ... See more keywords
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Effects of the magnetic field and zinc isotopes on the colony forming ability and elemental composition of E. coli bacterial cells

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Published in 2018 at "Russian Chemical Bulletin"

DOI: 10.1007/s11172-018-2283-4

Abstract: The combined effects of external weak magnetic fields and nuclear spins of the magnetic isotope 67Zn on vital functions of E. coli bacterial cells were proved experimentally. Metabolism of the major elements in E. coli… read more here.

Keywords: coli bacterial; bacterial cells; field; field zinc ... See more keywords
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Exploring electroactive microenvironments in polymer-based nanocomposites to sensitize bacterial cells to low-dose embedded silver nanoparticles.

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Published in 2021 at "Acta biomaterialia"

DOI: 10.1016/j.actbio.2021.07.067

Abstract: The search for alternative antimicrobial strategies capable of avoiding resistance mechanisms in bacteria are highly needed due to the alarming emergence of antimicrobial resistance. The application of physical stimuli as a mean of sensitizing bacteria… read more here.

Keywords: bacterial cells; silver nanoparticles; low dose; electroactive microenvironments ... See more keywords
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Multimetal tolerance mechanisms in bacteria: The resistance strategies acquired by bacteria that can be exploited to 'clean-up' heavy metal contaminants from water.

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Published in 2019 at "Aquatic toxicology"

DOI: 10.1016/j.aquatox.2019.04.011

Abstract: Heavy metal pollution is one of the major environmental concerns worldwide. Toxic heavy metals when untreated get accumulated in environment and can pose severe threats to living organisms. It is well known that metals play… read more here.

Keywords: bacterial cells; multimetal tolerance; metal; heavy metals ... See more keywords
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Direct measurements of erythromycin's effect on protein synthesis kinetics in living bacterial cells.

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Published in 2021 at "Journal of molecular biology"

DOI: 10.1016/j.jmb.2021.166942

Abstract: Macrolide antibiotics, such as erythromycin, bind to the nascent peptide exit tunnel (NPET) of the bacterial ribosome and modulate protein synthesis depending on the nascent peptide sequence. Whereas in vitro biochemical and structural methods have… read more here.

Keywords: protein; bacterial cells; erythromycin effect; protein synthesis ... See more keywords
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Culture independent DNA extraction method for bacterial cells concentrated from water

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Published in 2022 at "MethodsX"

DOI: 10.1016/j.mex.2022.101653

Abstract: • To bypass the problem of viable but non-culturable bacteria that cannot be isolated by culturable methods would be to isolate DNA from bacterial cells concentrated from water samples used as a template for the… read more here.

Keywords: dna; dna extraction; method; water ... See more keywords
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Stochastic response of bacterial cells to antibiotics: its mechanisms and implications for population and evolutionary dynamics.

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Published in 2021 at "Current opinion in microbiology"

DOI: 10.1016/j.mib.2021.07.002

Abstract: The effectiveness of antibiotics against bacterial infections has been declining due to the emergence of resistance. Precisely understanding the response of bacteria to antibiotics is critical to maximizing antibiotic-induced bacterial eradication while minimizing the emergence… read more here.

Keywords: bacterial cells; stochastic response; response; cells antibiotics ... See more keywords
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Most probable number - loop mediated isothermal amplification (MPN-LAMP) for quantifying waterborne pathogens in

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Published in 2017 at "Journal of microbiological methods"

DOI: 10.1016/j.mimet.2016.11.010

Abstract: We are reporting a most probable number approach integrated to loop mediated isothermal technique (MPN-LAMP) focusing on Gram-negative Escherichia coli and Gram-positive Enterococcus faecalis bacterial cells without nucleic acids extraction. LAMP assays for uidA from… read more here.

Keywords: bacterial cells; lamp; probable number; mpn ... See more keywords