Generating Libraries of Random lacZY or GFP Gene Fusions in the Bacterial Chromosome: Screening for Differentially Expressed Fusions.
Sign Up to like & getrecommendations! Published in 2023 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot108196
Abstract: Transposable elements engineered to generate random gene fusions in the bacterial chromosome are valuable tools in the study of gene expression. In this protocol, we describe the use of a new series of transposons designed… read more here.
Keywords: gene fusions; transposition; fusions bacterial; bacterial chromosome ... See more keywords
EVR: reconstruction of bacterial chromosome 3D structure models using error-vector resultant algorithm
Sign Up to like & getrecommendations! Published in 2019 at "BMC Genomics"
DOI: 10.1186/s12864-019-6096-0
Abstract: BackgroundMore and more 3C/Hi-C experiments on prokaryotes have been published. However, most of the published modeling tools for chromosome 3D structures are targeting at eukaryotes. How to transform prokaryotic experimental chromosome interaction data into spatial… read more here.
Keywords: error vector; bacterial chromosome; chromosome structure; structure ... See more keywords