Paramagnetic spin labeling of a bacterial DnaB helicase for solid-state NMR.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of magnetic resonance"
DOI: 10.1016/j.jmr.2021.107075
Abstract: Labeling of biomolecules with a paramagnetic probe for nuclear magnetic resonance (NMR) spectroscopy enables determining long-range distance restraints, which are otherwise not accessible by classically used dipolar coupling-based NMR approaches. Distance restraints derived from paramagnetic… read more here.
Keywords: solid state; bacterial dnab; state nmr; dnab helicase ... See more keywords
Spectroscopic glimpses of the transition state of ATP hydrolysis trapped in a bacterial DnaB helicase
Sign Up to like & getrecommendations! Published in 2021 at "Nature Communications"
DOI: 10.1038/s41467-021-25599-z
Abstract: The ATP hydrolysis transition state of motor proteins is a weakly populated protein state that can be stabilized and investigated by replacing ATP with chemical mimics. We present atomic-level structural and dynamic insights on a… read more here.
Keywords: state; bacterial dnab; atp hydrolysis; transition state ... See more keywords