Biocatalytic synthesis of 2′‐deoxynucleotide 5′‐triphosphates from bacterial genomic DNA: Proof of principle
Sign Up to like & getrecommendations! Published in 2023 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.28374
Abstract: 2′‐deoxynucleoside 5′‐triphosphates (dNTPs) are the building blocks of DNA and are key reagents which are incorporated by polymerase enzymes during nucleic acid amplification techniques, such as polymerase chain reaction (PCR). These techniques are of high… read more here.
Keywords: proof principle; dna; genomic dna; bacterial genomic ... See more keywords
Fast and flexible bacterial genomic epidemiology with PopPUNK.
Sign Up to like & getrecommendations! Published in 2019 at "Genome research"
DOI: 10.1101/gr.241455.118
Abstract: The routine use of genomics for disease surveillance provides the opportunity for high-resolution bacterial epidemiology. Current whole-genome clustering and multilocus typing approaches do not fully exploit core and accessory genomic variation, and they cannot both… read more here.
Keywords: flexible bacterial; bacterial genomic; genomic epidemiology; epidemiology ... See more keywords
Preparing Bacterial Genomic DNA.
Sign Up to like & getrecommendations! Published in 2022 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot107853
Abstract: We describe two alternative procedures for purifying bacterial chromosomal DNA. The first procedure incorporates the use of a commercial kit based on silica membrane technology. This approach relies on the selective binding of DNA to… read more here.
Keywords: dna; genomic dna; bacterial genomic; procedure ... See more keywords
Amplification of bacterial genomic DNA from all ascitic fluids with a highly sensitive polymerase chain reaction
Sign Up to like & getrecommendations! Published in 2018 at "Molecular Medicine Reports"
DOI: 10.3892/mmr.2018.9159
Abstract: Due to varying positive rates of polymerase chain reaction (PCR) amplification, interpretation of conventional PCR results for non-infectious ascites remains problematic. The present study developed a highly sensitive PCR protocol and investigated the positive rate… read more here.
Keywords: bacterial genomic; dna; genomic dna; pcr ... See more keywords