Knockout of Sf‐Caspase‐1 generates apoptosis‐resistant Sf9 cell lines: Implications for baculovirus expression
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology Journal"
DOI: 10.1002/biot.202100532
Abstract: The Sf9 cell line, originally isolated from the insect Spodoptera frugiperda, is commonly used alongside the baculovirus expression vector system (BEVS) to produce recombinant proteins and other biologics. As more BEVS‐derived vaccines and therapeutics are… read more here.
Keywords: cell; caspase; sf9 cell; baculovirus expression ... See more keywords
Expression and Purification of SH2 Domains Using Baculovirus Expression System.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6762-9_11
Abstract: Recombinant proteins expressed in bacteria are sometimes insoluble, aggregated, and incorrectly folded. For those Src homology 2 (SH2) domains that are insoluble in bacteria, baculovirus-insect cell expression systems can be an alternative to produce soluble… read more here.
Keywords: sh2 domains; domains using; baculovirus expression; expression system ... See more keywords
Effect of Temporal Expression of Integral Membrane Proteins by Baculovirus Expression Vector System
Sign Up to like & getrecommendations! Published in 2018 at "Molecular Biotechnology"
DOI: 10.1007/s12033-018-0099-y
Abstract: Integral membrane proteins (IMPs) are popular target for drugs, but their resolved structures have been overlooked when compared with cytosolic proteins. The main reason is that IMPs usually need intensive post-translational modifications and they are… read more here.
Keywords: system; membrane proteins; integral membrane; baculovirus expression ... See more keywords
Development of a novel enhanced baculovirus expression vector via promoter combination
Sign Up to like & getrecommendations! Published in 2020 at "Journal of Asia-pacific Entomology"
DOI: 10.1016/j.aspen.2020.07.016
Abstract: Abstract Baculovirus expression systems (BESs) have been used to express many foreign proteins. BESs are known to be very suitable for foreign protein production due to the strong gene expression ability of the polyhedrin promoter.… read more here.
Keywords: polyhedrin promoter; vector; production; baculovirus expression ... See more keywords
Recombinant expression of extracellular domain of mutant Epidermal Growth Factor Receptor in prokaryotic and baculovirus expression systems.
Sign Up to like & getrecommendations! Published in 2018 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2017.12.145
Abstract: Epidermal Growth Factor Receptor variant III (EGFRvIII) is a tumor specific antigen detected in various tumors including gliomas, breast cancer, lung cancer, head and neck squamous cell carcinoma (HNSCC). Screening of EGFRvIII targeting drug molecules… read more here.
Keywords: system; baculovirus expression; factor receptor; growth factor ... See more keywords
In vivo enzymatic digestion of HRV 3C protease cleavage sites-containing proteins produced in a silkworm-baculovirus expression system
Sign Up to like & getrecommendations! Published in 2022 at "Bioscience Reports"
DOI: 10.1042/bsr20220739
Abstract: Abstract Baculovirus expression vector system (BEVS) has been recognized as a potent protein expression system in engineering valuable enzymes and vaccines. Various fusion tags facilitate protein purification, leaving the potential risk to influence the target… read more here.
Keywords: system; baculovirus expression; cleavage; protease ... See more keywords
Expression and purification of MERS-CoV envelope protein, an essential viroporin, using the baculovirus expression system
Sign Up to like & getrecommendations! Published in 2023 at "Iranian Journal of Microbiology"
DOI: 10.18502/ijm.v15i1.11926
Abstract: Background and Objectives: The causative agent of Middle East Respiratory Syndrome (MERS) is a zoonotic Coronavirus (MERS-CoV) identified in Saudi Arabia in 2012. The envelope (E) protein of MERS-CoV is a small viral protein which… read more here.
Keywords: baculovirus expression; envelope protein; expression; mers cov ... See more keywords
Successful Rescue of Synthetic AcMNPV with a ~17 kb Deletion in the C1 Region of the Genome
Sign Up to like & getrecommendations! Published in 2022 at "Viruses"
DOI: 10.3390/v14122780
Abstract: Baculoviruses have been widely used as expression vectors. However, numerous genes in the baculoviral genome are non-essential for cellular infection and protein expression, making the optimisation of baculovirus expression vectors possible. We used a synthetic… read more here.
Keywords: baculovirus expression; region; genome; expression vectors ... See more keywords