Characterizing single extracellular vesicles by droplet barcode sequencing for protein analysis
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Extracellular Vesicles"
DOI: 10.1002/jev2.12277
Abstract: Abstract Small extracellular vesicles (sEVs) have in recent years evolved as a source of biomarkers for disease diagnosis and therapeutic follow up. sEV samples derived from multicellular organisms exhibit a high heterogeneous repertoire of vesicles… read more here.
Keywords: droplet barcode; extracellular vesicles; protein analysis; barcode sequencing ... See more keywords
Dual-molecular barcode sequencing detects rare variants in tumor and cell free DNA in plasma
Sign Up to like & getrecommendations! Published in 2020 at "Scientific Reports"
DOI: 10.1038/s41598-020-60361-3
Abstract: Conventional next generation sequencing analysis has provided important insights into cancer genetics. However, the detection of rare (low allele fraction) variants remains difficult because of the error-prone nucleotide changes derived from sequencing/PCR errors. To eliminate… read more here.
Keywords: dna; barcode; molecular barcode; barcode sequencing ... See more keywords
Efficient in situ barcode sequencing using padlock probe-based BaristaSeq
Sign Up to like & getrecommendations! Published in 2018 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkx1206
Abstract: Abstract Cellular DNA/RNA tags (barcodes) allow for multiplexed cell lineage tracing and neuronal projection mapping with cellular resolution. Conventional approaches to reading out cellular barcodes trade off spatial resolution with throughput. Bulk sequencing achieves high… read more here.
Keywords: resolution; padlock probe; barcode sequencing; probe based ... See more keywords
Applications of molecular barcode sequencing for the detection of low‐frequency variants in circulating tumour DNA from hepatocellular carcinoma
Sign Up to like & getrecommendations! Published in 2022 at "Liver International"
DOI: 10.1111/liv.15356
Abstract: Liquid biopsy has emerged as a promising tool for minimally invasive and accurate detection of various malignancies. We aimed to apply molecular barcode sequencing to circulating tumour DNA (ctDNA) from liquid biopsies of hepatocellular carcinoma… read more here.
Keywords: tumour dna; hepatocellular carcinoma; circulating tumour; molecular barcode ... See more keywords