Pioneer Factor Improves CRISPR‐Based C‐To‐G and C‐To‐T Base Editing
Sign Up to like & getrecommendations! Published in 2022 at "Advanced Science"
DOI: 10.1002/advs.202202957
Abstract: Base editing events in eukaryote require a compatible chromatin environment, but there is little research on how chromatin factors contribute to the editing efficiency or window. By engineering BEs (base editors) fused with various pioneer… read more here.
Keywords: editing efficiency; pioneer; base editing; efficiency ... See more keywords
Highly efficient CRISPR-mediated base editing for the gut Bacteroides spp. with pnCasBS-CBE.
Sign Up to like & getrecommendations! Published in 2023 at "Biotechnology journal"
DOI: 10.1002/biot.202200504
Abstract: Bacteroidales are the most abundant order of bacteria in the healthy human gut and have the potential as a therapeutic agent. We constructed a pnCasBS-CBE system for base editing in the Bacteroides thetaiotaomicron to expand… read more here.
Keywords: system; gut bacteroides; cbe; base editing ... See more keywords
Precise A∙T to G∙C base editing in the allotetraploid rapeseed (Brassica napus L.) genome
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Cellular Physiology"
DOI: 10.1002/jcp.30904
Abstract: Rapeseed is an important source of oilseed crop in the world. Achieving genetic improvement has always been the major goal in rapeseed production. Single nucleotide substitution is the basis of most genetic variation underpinning important… read more here.
Keywords: editing efficiency; precise base; brassica napus; rapeseed ... See more keywords
CRISPR Base Editing in Induced Pluripotent Stem Cells.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/7651_2019_243
Abstract: Induced pluripotent stem cells (iPSCs) have demonstrated tremendous potential in numerous disease modeling and regenerative medicine-based therapies. The development of innovative gene transduction and editing technologies has further augmented the potential of iPSCs. Cas9-cytidine deaminases,… read more here.
Keywords: base editing; stem cells; pluripotent stem; induced pluripotent ... See more keywords
Expanded targeting scope and enhanced base editing efficiency in rabbit using optimized xCas9(3.7)
Sign Up to like & getrecommendations! Published in 2019 at "Cellular and Molecular Life Sciences"
DOI: 10.1007/s00018-019-03110-8
Abstract: Evolved xCas9(3.7) variant with broad PAM compatibility has been reported in cell lines, while its editing efficiency was site-specific. Here, we show that xCas9(3.7) can recognize a broad PAMs including NGG, NGA, and NGT, in… read more here.
Keywords: base editing; optimized xcas9; base; editing efficiency ... See more keywords
The Solanum tuberosum GBSSI gene: a target for assessing gene and base editing in tetraploid potato
Sign Up to like & getrecommendations! Published in 2019 at "Plant Cell Reports"
DOI: 10.1007/s00299-019-02426-w
Abstract: AbstractKey MessageThe StGBSSI gene was successfully and precisely edited in the tetraploid potato using gene and base-editing strategies, leading to plants with impaired amylose biosynthesis.AbstractGenome editing has recently become a method of choice for basic… read more here.
Keywords: base editing; base; tetraploid potato; gene ... See more keywords
Base editing in rice: current progress, advances, limitations, and future perspectives.
Sign Up to like & getrecommendations! Published in 2021 at "Plant cell reports"
DOI: 10.1007/s00299-020-02656-3
Abstract: KEY MESSAGE Base editing is one of the promising genome editing tools for generating single-nucleotide changes in rice genome. Rice (Oryza sativa L.) is an important staple food crop, feeding half of the population around… read more here.
Keywords: base editing; genome editing; editing rice; rice ... See more keywords
Efficient base editing in tomato using a highly expressed transient system.
Sign Up to like & getrecommendations! Published in 2021 at "Plant cell reports"
DOI: 10.1007/s00299-021-02662-z
Abstract: KEY MESSAGE Base editing in tomatoes was achieved by transient expression. The Solanaceae plants, particularly the tomato (Solanum lycopersicum), is of huge economic value worldwide. The tomato is a unique model plant for studying the… read more here.
Keywords: base editing; transient expression; base; system ... See more keywords
Determinants of Base Editing Outcomes from Target Library Analysis and Machine Learning
Sign Up to like & getrecommendations! Published in 2020 at "Cell"
DOI: 10.1016/j.cell.2020.05.037
Abstract: Although base editors are widely used to install targeted point mutations, the factors that determine base editing outcomes are not well understood. We characterized sequence-activity relationships of 11 cytosine and adenine base editors (CBEs and… read more here.
Keywords: base editing; editing outcomes; machine learning; base editors ... See more keywords
Highly efficient CRISPR-SaKKH tools for plant multiplex cytosine base editing
Sign Up to like & getrecommendations! Published in 2020 at "Crop Journal"
DOI: 10.1016/j.cj.2020.03.002
Abstract: Abstract Base editing, as an expanded clustered regularly interspaced short palindromic repeats (CRISPR)-Cas genome editing strategy, permits precise and irreversible nucleotide conversion. SaKKH, an efficient variant of a Cas9 ortholog from Staphylococcus aureus (SaCas9), is… read more here.
Keywords: base editing; sakkh; base; multiplex cytosine ... See more keywords
Highly efficient single base editing in Aspergillus niger with CRISPR/Cas9 cytidine deaminase fusion.
Sign Up to like & getrecommendations! Published in 2019 at "Microbiological research"
DOI: 10.1016/j.micres.2019.03.007
Abstract: Classic genome editing tools including ZFN, TALEN, and CRISPR/Cas9 rely on DNA double-strand breaks for genome editing. To prevent the potential hazard caused by double-strand breaks (DSBs), a series of single base editing tools that… read more here.
Keywords: aspergillus niger; single base; base; crispr cas9 ... See more keywords