A modified glycosylase base editor without predictable DNA off‐target effects
Sign Up to like & getrecommendations! Published in 2024 at "FEBS Letters"
DOI: 10.1002/1873-3468.14970
Abstract: Glycosylase base editor (GBE) can induce C‐to‐G transversion in mammalian cells, showing great promise for the treatment of human genetic disorders. However, the limited efficiency of transversion and the possibility of off‐target effects caused by… read more here.
Keywords: dna target; target effects; glycosylase base; base editor ... See more keywords
Develop a Compact RNA Base Editor by Fusing ADAR with Engineered EcCas6e.
Sign Up to like & getrecommendations! Published in 2023 at "Advanced science"
DOI: 10.1002/advs.202206813
Abstract: Catalytically inactive CRISPR-Cas13 (dCas13)-based base editors can achieve the conversion of adenine-to-inosine (A-to-I) or cytidine-to-uridine (C-to-U) at the RNA level, however, the large size of dCas13 protein limits its in vivo applications. Here, a compact… read more here.
Keywords: compact rna; base editor; rna base; develop compact ... See more keywords
Efficient CRISPR‐mediated C‐to‐T base editing in Komagataella phaffii
Sign Up to like & getrecommendations! Published in 2024 at "Biotechnology Journal"
DOI: 10.1002/biot.202400115
Abstract: The nonconventional methylotrophic yeast Komagataella phaffii is widely applied in the production of industrial enzymes, pharmaceutical proteins, and various high‐value chemicals. The development of robust and versatile genome editing tools for K. phaffii is crucial… read more here.
Keywords: base editing; base; base editor; komagataella phaffii ... See more keywords
Designing an Elusive C•G→G•C CRISPR Base Editor.
Sign Up to like & getrecommendations! Published in 2019 at "Trends in biochemical sciences"
DOI: 10.1016/j.tibs.2018.10.004
Abstract: Protein engineering advances, including DNA repair manipulation of CRISPR (Clustered Regularly Interspaced Short Palindromic Repeat) machinery, have paved the way for the first set of DNA precision base editors (C•G→T•A and A•T→G•C), with wide-ranging implications… read more here.
Keywords: designing elusive; base; elusive crispr; base editor ... See more keywords
Biomolecular Insights into Extracellular Pollutant Reduction Pathways of Geobacter sulfurreducens Using a Base Editor System.
Sign Up to like & getrecommendations! Published in 2022 at "Environmental science & technology"
DOI: 10.1021/acs.est.2c02756
Abstract: Geobacter species are critically involved in elemental biogeochemical cycling and environmental bioremediation processes via extracellular electron transfer (EET), but the underlying biomolecular mechanisms remain elusive due to lack of effective analytical tools to explore into… read more here.
Keywords: geobacter; reduction; geobacter sulfurreducens; base editor ... See more keywords
Chemical modifications of adenine base editor mRNA and guide RNA expand its application scope
Sign Up to like & getrecommendations! Published in 2020 at "Nature Communications"
DOI: 10.1038/s41467-020-15892-8
Abstract: CRISPR-Cas9-associated base editing is a promising tool to correct pathogenic single nucleotide mutations in research or therapeutic settings. Efficient base editing requires cellular exposure to levels of base editors that can be difficult to attain… read more here.
Keywords: guide rna; editor; base; base editor ... See more keywords
Engineered cytosine base editor enabling broad-scope and high-fidelity gene editing in Streptomyces
Sign Up to like & getrecommendations! Published in 2024 at "Nature Communications"
DOI: 10.1038/s41467-024-49987-3
Abstract: Base editing (BE) faces protospacer adjacent motif (PAM) constraints and off-target effects in both eukaryotes and prokaryotes. For Streptomyces, renowned as one of the most prolific bacterial producers of antibiotics, the challenges are more pronounced… read more here.
Keywords: fidelity; base; base editor; escbe3 hypa ... See more keywords
Dual base editor catalyzes both cytosine and adenine base conversions in human cells
Sign Up to like & getrecommendations! Published in 2020 at "Nature Biotechnology"
DOI: 10.1038/s41587-020-0527-y
Abstract: Although base editors are useful tools for precise genome editing, current base editors can only convert either adenines or cytosines. We developed a dual adenine and cytosine base editor (A&C-BEmax) by fusing both deaminases with… read more here.
Keywords: dual base; base editors; editor catalyzes; base ... See more keywords
An ADAR2-mimic base editor for efficient C-to-U RNA editing in vivo.
Sign Up to like & getrecommendations! Published in 2025 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2505269122
Abstract: RNA base editing has the capability to rewrite genetic codes while offering improved safety due to its reversibility and temporal-spatial tunability. The development of cytosine-to-uridine editors faces issues of DNA mutagenic activity of APOBECs and… read more here.
Keywords: mimic base; rna editing; rna; base editor ... See more keywords
DNA capture by a CRISPR-Cas9–guided adenine base editor
Sign Up to like & getrecommendations! Published in 2020 at "Science"
DOI: 10.1126/science.abb1390
Abstract: Secrets of a fast base editor CRISPR-Cas9 base editors comprise RNA-guided Cas proteins fused to an enzyme that can deaminate a DNA nucleoside. No natural enzyme deaminates adenine in DNA, and so a breakthrough came… read more here.
Keywords: crispr cas9; base; base editor; dna ... See more keywords
Rescue of autosomal dominant hearing loss by in vivo delivery of mini dCas13X-derived RNA base editor
Sign Up to like & getrecommendations! Published in 2022 at "Science Translational Medicine"
DOI: 10.1126/scitranslmed.abn0449
Abstract: Programmable RNA editing tools enable the reversible correction of mutant transcripts, reducing the potential risk associated with permanent genetic changes associated with the use of DNA editing tools. However, the potential of these RNA tools… read more here.
Keywords: rna editing; hearing loss; base editor; rna base ... See more keywords