Develop a Compact RNA Base Editor by Fusing ADAR with Engineered EcCas6e.
Sign Up to like & getrecommendations! Published in 2023 at "Advanced science"
DOI: 10.1002/advs.202206813
Abstract: Catalytically inactive CRISPR-Cas13 (dCas13)-based base editors can achieve the conversion of adenine-to-inosine (A-to-I) or cytidine-to-uridine (C-to-U) at the RNA level, however, the large size of dCas13 protein limits its in vivo applications. Here, a compact… read more here.
Keywords: compact rna; base editor; rna base; develop compact ... See more keywords
Designing an Elusive C•G→G•C CRISPR Base Editor.
Sign Up to like & getrecommendations! Published in 2019 at "Trends in biochemical sciences"
DOI: 10.1016/j.tibs.2018.10.004
Abstract: Protein engineering advances, including DNA repair manipulation of CRISPR (Clustered Regularly Interspaced Short Palindromic Repeat) machinery, have paved the way for the first set of DNA precision base editors (C•G→T•A and A•T→G•C), with wide-ranging implications… read more here.
Keywords: designing elusive; base; elusive crispr; base editor ... See more keywords
Biomolecular Insights into Extracellular Pollutant Reduction Pathways of Geobacter sulfurreducens Using a Base Editor System.
Sign Up to like & getrecommendations! Published in 2022 at "Environmental science & technology"
DOI: 10.1021/acs.est.2c02756
Abstract: Geobacter species are critically involved in elemental biogeochemical cycling and environmental bioremediation processes via extracellular electron transfer (EET), but the underlying biomolecular mechanisms remain elusive due to lack of effective analytical tools to explore into… read more here.
Keywords: geobacter; reduction; geobacter sulfurreducens; base editor ... See more keywords
Chemical modifications of adenine base editor mRNA and guide RNA expand its application scope
Sign Up to like & getrecommendations! Published in 2020 at "Nature Communications"
DOI: 10.1038/s41467-020-15892-8
Abstract: CRISPR-Cas9-associated base editing is a promising tool to correct pathogenic single nucleotide mutations in research or therapeutic settings. Efficient base editing requires cellular exposure to levels of base editors that can be difficult to attain… read more here.
Keywords: guide rna; editor; base; base editor ... See more keywords
Dual base editor catalyzes both cytosine and adenine base conversions in human cells
Sign Up to like & getrecommendations! Published in 2020 at "Nature Biotechnology"
DOI: 10.1038/s41587-020-0527-y
Abstract: Although base editors are useful tools for precise genome editing, current base editors can only convert either adenines or cytosines. We developed a dual adenine and cytosine base editor (A&C-BEmax) by fusing both deaminases with… read more here.
Keywords: dual base; base editors; editor catalyzes; base ... See more keywords
DNA capture by a CRISPR-Cas9–guided adenine base editor
Sign Up to like & getrecommendations! Published in 2020 at "Science"
DOI: 10.1126/science.abb1390
Abstract: Secrets of a fast base editor CRISPR-Cas9 base editors comprise RNA-guided Cas proteins fused to an enzyme that can deaminate a DNA nucleoside. No natural enzyme deaminates adenine in DNA, and so a breakthrough came… read more here.
Keywords: crispr cas9; base; base editor; dna ... See more keywords
Rescue of autosomal dominant hearing loss by in vivo delivery of mini dCas13X-derived RNA base editor
Sign Up to like & getrecommendations! Published in 2022 at "Science Translational Medicine"
DOI: 10.1126/scitranslmed.abn0449
Abstract: Programmable RNA editing tools enable the reversible correction of mutant transcripts, reducing the potential risk associated with permanent genetic changes associated with the use of DNA editing tools. However, the potential of these RNA tools… read more here.
Keywords: rna editing; hearing loss; base editor; rna base ... See more keywords
Genome Editing of Veterinary Relevant Mycoplasmas Using a CRISPR-Cas Base Editor System
Sign Up to like & getrecommendations! Published in 2022 at "Applied and Environmental Microbiology"
DOI: 10.1128/aem.00996-22
Abstract: Mycoplasmas are minimal pathogenic bacteria that infect a wide range of hosts, including humans, livestock, and wild animals. Major pathogenic species cause acute to chronic infections involving still poorly characterized virulence factors. ABSTRACT Mycoplasmas are… read more here.
Keywords: system; mycoplasmas; base editor; virulence factors ... See more keywords
ACBE, a new base editor for simultaneous C-to-T and A-to-G substitutions in mammalian systems
Sign Up to like & getrecommendations! Published in 2020 at "BMC Biology"
DOI: 10.1186/s12915-020-00866-5
Abstract: Background Many favorable traits of crops and livestock and human genetic diseases arise from multiple single nucleotide polymorphisms or multiple point mutations with heterogeneous base substitutions at the same locus. Current cytosine or adenine base… read more here.
Keywords: new base; point mutations; base; base editor ... See more keywords
Genome-wide base editor screen identifies regulators of protein abundance in yeast
Sign Up to like & getrecommendations! Published in 2022 at "eLife"
DOI: 10.7554/elife.79525
Abstract: Abundance of proteins is extensively regulated both transcriptionally and post-transcriptionally. To systematically characterize how regulation of protein abundance is encoded in the genome and identify protein regulators on a genome-wide scale, we developed a genetic… read more here.
Keywords: genome wide; abundance; base editor; protein abundance ... See more keywords