Cas9‐orthologue‐mediated cytosine and adenine base editors recognizing NNAAAA PAM sequences
Sign Up to like & getrecommendations! Published in 2023 at "Biotechnology Journal"
DOI: 10.1002/biot.202200533
Abstract: CRISPR/Cas9 system has been applied as an effective genome‐targeting technology. By fusing deaminases with Cas9 nickase (nCas9), various cytosine and adenine base editors (CBEs and ABEs) have been successfully developed that can efficiently induce nucleotide… read more here.
Keywords: cytosine adenine; pam sequences; base editors; adenine base ... See more keywords
Off-target effects of base editors: what we know and how we can reduce it.
Sign Up to like & getrecommendations! Published in 2021 at "Current genetics"
DOI: 10.1007/s00294-021-01211-1
Abstract: The recently discovered CRISPR-Cas9 modification, base editors (BEs), is considered as one of the most promising tools for correcting disease-causing mutations in humans, since it allows point substitutions to be edited without generating double-stranded DNA… read more here.
Keywords: target effects; effects base; genetics; base editors ... See more keywords
Determinants of Base Editing Outcomes from Target Library Analysis and Machine Learning
Sign Up to like & getrecommendations! Published in 2020 at "Cell"
DOI: 10.1016/j.cell.2020.05.037
Abstract: Although base editors are widely used to install targeted point mutations, the factors that determine base editing outcomes are not well understood. We characterized sequence-activity relationships of 11 cytosine and adenine base editors (CBEs and… read more here.
Keywords: base editing; editing outcomes; machine learning; base editors ... See more keywords
Rationally Designed APOBEC3B Cytosine Base Editors with Improved Specificity.
Sign Up to like & getrecommendations! Published in 2020 at "Molecular cell"
DOI: 10.1016/j.molcel.2020.07.005
Abstract: Cytosine base editors (CBEs) generate C-to-T nucleotide substitutions in genomic target sites without inducing double-strand breaks. However, CBEs such as BE3 can cause genome-wide off-target changes via sgRNA-independent DNA deamination. By leveraging the orthogonal R-loops… read more here.
Keywords: base editors; target; base; cytosine base ... See more keywords
Directed Evolution of CRISPR-Cas9 Base Editors.
Sign Up to like & getrecommendations! Published in 2019 at "Trends in biotechnology"
DOI: 10.1016/j.tibtech.2019.09.005
Abstract: A recent publication by Thuronyi et al. described a directed evolution system called phage-assisted continuous evolution (PACE) that was used to generate improved variants of CRISPR-Cas9 base editors. These evolved base editors overcome some of the… read more here.
Keywords: evolution; crispr cas9; base editors; directed evolution ... See more keywords
Genome, Epigenome, and Transcriptome Editing via Chemical Modification of Nucleobases in Living Cells.
Sign Up to like & getrecommendations! Published in 2019 at "Biochemistry"
DOI: 10.1021/acs.biochem.8b00958
Abstract: Base editors are tools that chemically modify the nucleobases of DNA and RNA in a programmable manner, allowing for genome, epigenome, and transcriptome editing in live cells. These tools can be used to introduce specific… read more here.
Keywords: transcriptome editing; base editors; genome epigenome; editing via ... See more keywords
Off-target Editing by CRISPR-guided DNA base editors.
Sign Up to like & getrecommendations! Published in 2019 at "Biochemistry"
DOI: 10.1021/acs.biochem.9b00573
Abstract: Base editing is a genome editing strategy that induces specific single nucleotide changes within genomic DNA. Two major DNA base editors, Cytosine base editors (CBEs) and Adenine base editors (ABEs), have been developed that consist… read more here.
Keywords: crispr; base editors; target editing; dna base ... See more keywords
Optimized base editors enable efficient editing in cells, organoids and mice
Sign Up to like & getrecommendations! Published in 2018 at "Nature biotechnology"
DOI: 10.1038/nbt.4194
Abstract: CRISPR base editing enables the creation of targeted single-base conversions without generating double-stranded breaks. However, the efficiency of current base editors is very low in many cell types. We reengineered the sequences of BE3, BE4Gam,… read more here.
Keywords: enable efficient; editors enable; base editors; base ... See more keywords
BE-PIGS: a base-editing tool with deaminases inlaid into Cas9 PI domain significantly expanded the editing scope
Sign Up to like & getrecommendations! Published in 2019 at "Signal Transduction and Targeted Therapy"
DOI: 10.1038/s41392-019-0072-7
Abstract: To the Editor, The discovery of base-editing tools sheds new light on both basic biomedical research and clinical therapeutics. Although showing profound performance in a wide range of applications, current base editors have their limitations.… read more here.
Keywords: window; base editors; base; cas9 ... See more keywords
Targeted exon skipping with AAV-mediated split adenine base editors
Sign Up to like & getrecommendations! Published in 2019 at "Cell Discovery"
DOI: 10.1038/s41421-019-0109-7
Abstract: Techniques for exclusion of exons from mature transcripts have been applied as gene therapies for treating many different diseases. Since exon skipping has been traditionally accomplished using technologies that have a transient effect, it is… read more here.
Keywords: targeted exon; base editors; adenine base; base ... See more keywords
Efficient base editing for multiple genes and loci in pigs using base editors
Sign Up to like & getrecommendations! Published in 2019 at "Nature Communications"
DOI: 10.1038/s41467-019-10421-8
Abstract: Cytosine base editors (CBEs) enable programmable C-to-T conversion without DNA double-stranded breaks and homology-directed repair in a variety of organisms, which exhibit great potential for agricultural and biomedical applications. However, all reported cases only involved… read more here.
Keywords: base editing; multiple sites; efficient base; editing multiple ... See more keywords