Real‐world clinical experience with serum MOG and AQP4 antibody testing by live versus fixed cell‐based assay
Sign Up to like & getrecommendations! Published in 2025 at "Annals of Clinical and Translational Neurology"
DOI: 10.1002/acn3.52310
Abstract: To assess the real‐world performance of a live (LCBA) versus a fixed (FCBA) cell‐based assay for the detection of serum antibodies directed against myelin oligodendrocyte glycoprotein (MOG‐IgG) and aquaporin‐4 (AQP4‐IgG). read more here.
Keywords: real world; versus fixed; cell based; based assay ... See more keywords
Microscopy‐based assay for semi‐quantitative detection of SARS‐CoV‐2 specific antibodies in human sera
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DOI: 10.1002/bies.202000257
Abstract: Emergence of the novel pathogenic coronavirus SARS‐CoV‐2 and its rapid pandemic spread presents challenges that demand immediate attention. Here, we describe the development of a semi‐quantitative high‐content microscopy‐based assay for detection of three major classes… read more here.
Keywords: microscopy; cov specific; microscopy based; sars cov ... See more keywords
A Fluorescence-Based Assay to Determine PDZ-Ligand Binding Thermodynamics.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1166-1_8
Abstract: Postsynaptic density-95, disks-large, and zonula occludens-1 (PDZ) domain interactions with cognate linear binding motifs (i.e., PDZ-binding motifs or PBMs) are important for many biological processes and can be pathological when disrupted. There are hundreds of… read more here.
Keywords: thermodynamics; fluorescence based; assay determine; pdz pbm ... See more keywords
Fluorescence-Based Assay For Measuring OMA1 Activity.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1266-8_24
Abstract: Mitochondrial fusion depends on proteolytic processing of the dynamin-related GTPase protein, OPA1, which is regulated by the mitochondrial zinc metalloproteinase, OMA1. Last year we published a report describing a novel approach to directly measure the… read more here.
Keywords: fluorescence based; oma1 activity; assay measuring; based assay ... See more keywords
Plate-Based Assay for Measuring Direct Semaphorin-Neuropilin Interactions.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6448-2_5
Abstract: The semaphorins are an essential family of axon guidance molecules that can be either secreted or are transmembrane proteins. Class 3 semaphorin (Sema3) family members are secreted and provide long-range guidance cues through two receptor… read more here.
Keywords: semaphorin; sema3 nrp; plate based; assay measuring ... See more keywords
A Cell-Based Assay for Mitotic Spindle Orientation.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7847-2_5
Abstract: The regulation of mitotic spindle orientation is essential to ensure proper cell division and development (Kiyomitsua and Cheeseman Nat Cell Biol 14:311-317, 2012). For identification of potential spindle orientation regulators, determination of the mitotic spindle… read more here.
Keywords: spindle orientation; cell based; mitotic spindle; based assay ... See more keywords
A model-based assay design to reproduce in vivo patterns of acute drug-induced toxicity
Sign Up to like & getrecommendations! Published in 2017 at "Archives of Toxicology"
DOI: 10.1007/s00204-017-2041-7
Abstract: Lars Kuepfer1 · Olivia Clayton2 · Christoph Thiel1 · Henrik Cordes1 · Ramona Nudischer2 · Lars M. Blank1 · Vanessa Baier1 · Stephane Heymans3,4 · Florian Caiment5 · Adrian Roth2 · David A. Fluri6 ·… read more here.
Keywords: model based; design reproduce; assay design; vivo patterns ... See more keywords
Adaptation and optimization of a fluorescence-based assay for in vivo antimalarial drug screening
Sign Up to like & getrecommendations! Published in 2017 at "Parasitology Research"
DOI: 10.1007/s00436-017-5477-z
Abstract: The in vivo efficacy of potential antimalarials is usually evaluated by direct microscopic determination of the parasitaemia of Plasmodium-infected mice on Giemsa-stained blood smears. This process is time-consuming, requires experienced technicians and is not automatable.… read more here.
Keywords: fluorescence; fluorescence based; antimalarial drug; based assay ... See more keywords
Development of a Cell-Based Assay to Assess Binding of the proNGF Prodomain to Sortilin
Sign Up to like & getrecommendations! Published in 2017 at "Cellular and Molecular Neurobiology"
DOI: 10.1007/s10571-017-0558-1
Abstract: Sortilin was first identified based on its activity as part of intracellular protein sorting machinery. Recently, it was discovered that sortilin also acts as a cell surface receptor for the propeptide form of nerve growth… read more here.
Keywords: sortilin; cell based; interaction; based assay ... See more keywords
Quantitative PCR for detection and quantification of Phytophthora cactorum in the cultivation of strawberry
Sign Up to like & getrecommendations! Published in 2021 at "European Journal of Plant Pathology"
DOI: 10.1007/s10658-021-02290-z
Abstract: In this study, we report on the development of two quantitative PCR assays for the detection and quantification of the soilborne plant pathogen Phytophthora cactorum causing root and crown rot of strawberry. The assays rely… read more here.
Keywords: plant; based assay; quantification; growth substrate ... See more keywords
Characterization of an archaeal inorganic pyrophosphatase from Sulfolobus islandicus using a [31P]-NMR-based assay.
Sign Up to like & getrecommendations! Published in 2021 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2021.11.019
Abstract: Inorganic pyrophosphatase catalyzes the conversion of pyrophosphate to phosphate and is often critical for driving reactions forward in cellular processes such as nucleic acid and protein synthesis. Commonly used methods for quantifying pyrophosphatase enzyme activity… read more here.
Keywords: 31p nmr; pyrophosphatase; sulfolobus islandicus; based assay ... See more keywords