Detection of gastroenteritis viruses among pediatric patients in Hiroshima Prefecture, Japan, between 2006 and 2013 using multiplex reverse transcription PCR‐based assays involving fluorescent dye‐labeled primers
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Medical Virology"
DOI: 10.1002/jmv.24714
Abstract: Multiplex reverse transcription (RT)‐polymerase chain reaction (PCR)‐based assays involving fluorescent dye‐labeled primers were modified to detect 10 types of gastroenteritis viruses by adding two further assays to a previously developed assay. Then, these assays were… read more here.
Keywords: based assays; involving fluorescent; assays involving; fluorescent dye ... See more keywords
Computational Design of Multiplex Oligonucleotide-Based Assays.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0822-7_2
Abstract: The success of any oligonucleotide-based experiment strongly depends on the accurate design of the components. Oli2go is a user-friendly web tool that provides efficient multiplex oligonucleotide design including specificity and primer dimer checks. Its fully… read more here.
Keywords: computational design; design multiplex; multiplex oligonucleotide; based assays ... See more keywords
Analyzing Trophoblast Function Using Cell-Based Assays.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7498-6_18
Abstract: Functional cell-based assays are useful for comparing the effect of a treatment, drug, or condition on cells in culture. Cell lines are a commonly used model to replicate a normal biological process or a pathological… read more here.
Keywords: analyzing trophoblast; based assays; cell based; function using ... See more keywords
Vesicle- and Hepatocyte-Based Assays for Identification of Drug Candidates Inhibiting BSEP Function.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9420-5_4
Abstract: Transporters play a crucial role in the uptake of endo- and exogenous molecules in hepatocytes and efflux into the bile. The bile salt export pump (BSEP; ABCB11) is of major importance for efflux of bile… read more here.
Keywords: bile salt; hepatocyte based; based assays; vesicle hepatocyte ... See more keywords
Cell-based assays as an alternative for the study of aquatic toxicity of pharmaceuticals
Sign Up to like & getrecommendations! Published in 2019 at "Environmental Science and Pollution Research"
DOI: 10.1007/s11356-019-07384-0
Abstract: An increasing number and amount of pharmaceuticals for human and veterinary use currently reach the aquatic environment, and the determination of their effects on aquatic organisms becomes of major importance. The 96-h fish lethal test… read more here.
Keywords: based assays; study; toxicity; cell based ... See more keywords
Yeast-based assays for detecting protein-protein/drug interactions and their inhibitors.
Sign Up to like & getrecommendations! Published in 2017 at "European journal of cell biology"
DOI: 10.1016/j.ejcb.2017.06.003
Abstract: Understanding cellular processes at molecular levels in health and disease requires the knowledge of protein-protein interactions (PPIs). In line with this, identification of PPIs at genome-wide scale is highly valuable to understand how different cellular… read more here.
Keywords: assays detecting; based assays; protein protein; protein drug ... See more keywords
Computational and Experimental Model to Study Immunobead-Based Assays in Microfluidic Mixing Platforms.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.1c04228
Abstract: In immunobead-based assays, micro/nanobeads are functionalized with antibodies to capture the target analytes, which can significantly improve the assay's performance. The immunobead-based assays have been recently combined with microfluidic mixing devices and customized for a… read more here.
Keywords: experimental model; computational experimental; microfluidic mixing; based assays ... See more keywords
Nanopatterned Extracellular Matrices Enable Cell-Based Assays with a Mass Spectrometric Readout
Sign Up to like & getrecommendations! Published in 2017 at "Nano Letters"
DOI: 10.1021/acs.nanolett.6b04176
Abstract: Cell-based assays are finding wider use in evaluating compounds in primary screens for drug development, yet it is still challenging to measure enzymatic activities as an end point in a cell-based assay. This paper reports… read more here.
Keywords: extracellular matrices; nanopatterned extracellular; matrices enable; based assays ... See more keywords
Epidermal growth factor receptor mutations in non–small cell lung cancer undetected by high-sensitivity allele-specific real-time polymerase chain reaction–based assays
Sign Up to like & getrecommendations! Published in 2020 at "Journal of the Chinese Medical Association"
DOI: 10.1097/jcma.0000000000000277
Abstract: Background: Identifying epidermal growth factor receptor (EGFR) mutation status is critical for planning lung cancer treatment. Sanger sequencing detects both known and novel mutations but shows poor sensitivity. High-sensitivity allele-specific real-time polymerase chain reaction (ASRP)-based… read more here.
Keywords: asrp based; based assays; lung cancer; sensitivity ... See more keywords
Measurement of tissue factor-positive extracellular vesicles in plasma: strengths and weaknesses of current methods
Sign Up to like & getrecommendations! Published in 2022 at "Current Opinion in Hematology"
DOI: 10.1097/moh.0000000000000730
Abstract: Purpose of review This review evaluates the different methods used to measure levels of tissue factor (TF) in plasma and on extracellular vesicles (EVs). Levels of TF-positive (TF+) EVs in blood are increased in a… read more here.
Keywords: based assays; measurement tissue; tissue factor; extracellular vesicles ... See more keywords
Evaluation of recombinant factor C assay for the detection of divergent lipopolysaccharide structural species and comparison with Limulus amebocyte lysate‐based assays and a human monocyte activity assay
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Medical Microbiology"
DOI: 10.1099/jmm.0.000510
Abstract: Purpose. The Limulus amebocytelysate (LAL) assay is widely used for the screening of lipopolysaccharide (LPS) in parenteral pharmaceuticals. However, correlation of LPS in Gram‐negative bacterial infections by LAL assay has been problematic, partly due to… read more here.
Keywords: monocyte activity; based assays; human monocyte; assays human ... See more keywords