Development of aptamer‐based ELISA method for d‐dimer detection
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology and Applied Biochemistry"
DOI: 10.1002/bab.2347
Abstract: This study developed an aptamer‐based enzyme‐linked immunosorbent assay (ELISA) system for sensitive detection of the d ‐dimer molecule, which is significantly elevated in several severe diseases. Aptamers are known to have advantages, such as longer… read more here.
Keywords: system; aptamer based; dimer; detection ... See more keywords
Development of a monoclonal-based ic-ELISA for the determination of kitasamycin in animal tissues and simulation studying its molecular recognition mechanism.
Sign Up to like & getrecommendations! Published in 2021 at "Food chemistry"
DOI: 10.1016/j.foodchem.2021.129465
Abstract: To monitor the residue of kitasamycin (KIT), a monoclonal antibody against KIT was prepared, and a 50% inhibition concentration (IC50) of 5.7 ± 1.4 μg/L was achieved with the most sensitive antibody, KA/2A9, by optimizing ELISA conditions. The… read more here.
Keywords: based elisa; animal tissues; development monoclonal; kitasamycin ... See more keywords
Evaluation of methods to reduce background using the Python-based ELISA_QC program.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of immunological methods"
DOI: 10.1016/j.jim.2018.02.013
Abstract: Almost all immunological approaches [immunohistochemistry, enzyme-linked immunosorbent assay (ELISA), Western blot], that are used to quantitate specific proteins have had to address high backgrounds due to non-specific reactivity. We report here for the first time… read more here.
Keywords: based elisa; elisa program; using python; python based ... See more keywords
Comparative diagnostic efficacy of recombinant LLO and PI-PLC-based ELISAs for detection of listeriosis in animals.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of microbiological methods"
DOI: 10.1016/j.mimet.2017.04.005
Abstract: The present study for the first time evaluates the serodiagnostic efficacy of two recombinant antigens namely, listeriolysin O (rLLO) and phosphatidyl-inositol phospholipase C (rPI-PLC). Indirect ELISA with the above recombinant antigens was used on samples… read more here.
Keywords: plc based; based elisa; seropositivity; rpi plc ... See more keywords
Improving the Sensitivity of Nanofibrous Membrane-Based ELISA for On-Site Antibiotics Detection.
Sign Up to like & getrecommendations! Published in 2022 at "ACS sensors"
DOI: 10.1021/acssensors.2c00208
Abstract: An ultrasensitive and portable colorimetric enzyme-linked immunosorbent assay (ELISA) sensor for antibiotics was fabricated by immobilizing antibodies inside the largely porous and highly hydrophilic nanofibrous membranes. Different from regular electrospun nanofibrous membranes where antibodies may… read more here.
Keywords: membrane based; based elisa; detection; nanofibrous membranes ... See more keywords
Improved assessment of accuracy and performance indicators in paper-based ELISA
Sign Up to like & getrecommendations! Published in 2017 at "Analytical Methods"
DOI: 10.1039/c7ay00505a
Abstract: Paper-based devices are an excellent match for low-cost point-of-care testing (POCT) tools. Their user-friendliness, portability, and short time of analysis, coupled with ease of local manufacture make these devices the best option for inexpensive diagnostic… read more here.
Keywords: paper based; based elisa; paper; accuracy ... See more keywords
A novel spike subunit 1-based ELISA reveals widespread porcine torovirus infection in eastern China.
Sign Up to like & getrecommendations! Published in 2021 at "Transboundary and emerging diseases"
DOI: 10.1111/tbed.14026
Abstract: Toroviruses (ToVs), closely related but genetically distinct from coronaviruses, are known to infect horses, cows, pigs, goats, and humans, mainly causing enteritic disorders. However, due to the lack of an adaptive culture system, porcine ToV… read more here.
Keywords: based elisa; eastern china; ptov; porcine ... See more keywords
A Multi-Epitope Fusion Protein-Based p-ELISA Method for Diagnosing Bovine and Goat Brucellosis
Sign Up to like & getrecommendations! Published in 2021 at "Frontiers in Veterinary Science"
DOI: 10.3389/fvets.2021.708008
Abstract: In recent years, the incidence of brucellosis has increased annually, causing tremendous economic losses to animal husbandry in a lot of countries. Therefore, developing rapid, sensitive, and specific diagnostic techniques is critical to control the… read more here.
Keywords: based elisa; brucellosis; bovine goat; fusion protein ... See more keywords
Development and Validation of a S1 Protein-Based ELISA for the Specific Detection of Antibodies against Equine Coronavirus
Sign Up to like & getrecommendations! Published in 2019 at "Viruses"
DOI: 10.3390/v11121109
Abstract: Equine coronavirus (ECoV) is considered to be involved in enteric diseases in foals. Recently, several outbreaks of ECoV infection have also been reported in adult horses from the USA, France and Japan. Epidemiological studies of… read more here.
Keywords: development validation; based elisa; ecov infection; equine coronavirus ... See more keywords
PEDV Infection Generates Conformation-Specific Antibodies That Can Be Effectively Detected by a Cell-Based ELISA
Sign Up to like & getrecommendations! Published in 2021 at "Viruses"
DOI: 10.3390/v13020303
Abstract: Porcine epidemic diarrhea virus (PEDV) is a coronavirus that causes serious and highly contagious enteric disease in swine worldwide. In this study, we constructed a recombinant baculovirus (S-Bac) expressing full-length spike protein of the virulent… read more here.
Keywords: pedv infection; based elisa; cell based; specific antibodies ... See more keywords