Cell-Based Enzyme-Linked Immunosorbent Assay (Cell-ELISA) Analysis of Native and Recombinant Glutamate Receptors.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9077-1_4
Abstract: Glutamate receptors (GluRs) located primarily on the membranes of neurons and glial cells are responsible for excitatory synaptic transmission in the central nervous system. The transport of GluRs to the cell surface is a highly… read more here.
Keywords: cell based; based enzyme; glutamate receptors; cell ... See more keywords
Colorimetric aptasensor for ochratoxin A detection based on enzyme-induced gold nanoparticle aggregation.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of hazardous materials"
DOI: 10.1016/j.jhazmat.2019.121758
Abstract: An innovative colorimetric method based on enzyme-induced gold nanoparticle aggregation was developed to detect the activity of alkaline phosphatase (ALP), and it was further applied to construct an aptasensor to monitor ochratoxin A (OTA) concentrations.… read more here.
Keywords: nanoparticle aggregation; based enzyme; detection; gold nanoparticle ... See more keywords
Ensemble-based enzyme design can recapitulate the effects of laboratory directed evolution in silico
Sign Up to like & getrecommendations! Published in 2020 at "Nature Communications"
DOI: 10.1038/s41467-020-18619-x
Abstract: The creation of artificial enzymes is a key objective of computational protein design. Although de novo enzymes have been successfully designed, these exhibit low catalytic efficiencies, requiring directed evolution to improve activity. Here, we use… read more here.
Keywords: evolution; enzyme design; based enzyme; directed evolution ... See more keywords
Rapid simultaneous extraction and magnetic particle-based enzyme immunoassay for the parallel determination of ochratoxin A, fumonisin B1 and deoxynivalenol mycotoxins in cereal samples
Sign Up to like & getrecommendations! Published in 2017 at "Analytical Methods"
DOI: 10.1039/c7ay00386b
Abstract: This work describes the development of a rapid method for the extraction and the immunochemical determination of three mycotoxins, deoxynivalenol, fumonisin B1, and ochratoxin A, which are frequently found together naturally in cereal samples (wheat… read more here.
Keywords: cereal samples; magnetic particle; determination; based enzyme ... See more keywords
Biosensor-based enzyme engineering approach applied to psicose biosynthesis
Sign Up to like & getrecommendations! Published in 2019 at "Synthetic Biology"
DOI: 10.1093/synbio/ysz028
Abstract: Abstract Bioproduction of chemical compounds is of great interest for modern industries, as it reduces their production costs and ecological impact. With the use of synthetic biology, metabolic engineering and enzyme engineering tools, the yield… read more here.
Keywords: engineering; based enzyme; biology; enzyme engineering ... See more keywords
Direct and Indirect Cell-Based Enzyme-Linked Immunosorbent Assay.
Sign Up to like & getrecommendations! Published in 2017 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot093732
Abstract: The direct cell-based enzyme-linked immunosorbent assay (ELISA) has developed into a popular alternative immunoassay for the rapid detection of expressed cell-surface antigens or receptors. It is used to determine cell surface antigen expression profiles using… read more here.
Keywords: linked immunosorbent; cell based; based enzyme; immunosorbent assay ... See more keywords