Assessing gene function in human B cells: CRISPR/Cas9‐based gene editing and mRNA‐based gene expression in healthy and tumor cells
Sign Up to like & getrecommendations! Published in 2022 at "European Journal of Immunology"
DOI: 10.1002/eji.202149784
Abstract: Robust protocols for genetic manipulation are highly needed to facilitate study of gene function in human B cells, both in settings of health and disease. B cells are notoriously hard to manipulate, being especially fragile… read more here.
Keywords: based gene; function human; human cells; gene function ... See more keywords
Omnibus testing approach for gene‐based gene‐gene interaction
Sign Up to like & getrecommendations! Published in 2022 at "Statistics in Medicine"
DOI: 10.1002/sim.9389
Abstract: Genetic interaction is considered as one of the main heritable component of complex traits. With the emergence of genome‐wide association studies (GWAS), a collection of statistical methods dedicated to the identification of interaction at the… read more here.
Keywords: based gene; interaction; gene gene; gene interaction ... See more keywords
CRISPR/Cas9-Based Gene Dropout Screens.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8876-1_15
Abstract: Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9-based technology enables efficient and precise perturbations of target genomic sites. Combining the endonuclease Cas9 and a pooled guide RNA library allows for systematic screenings of genes associated with… read more here.
Keywords: cas9 based; crispr cas9; gene dropout; dropout screens ... See more keywords
Exploring the role of peptides in polymer-based gene delivery.
Sign Up to like & getrecommendations! Published in 2017 at "Acta biomaterialia"
DOI: 10.1016/j.actbio.2017.07.043
Abstract: Polymers are widely studied as non-viral gene vectors because of their strong DNA binding ability, capacity to carry large payload, flexibility of chemical modifications, low immunogenicity, and facile processes for manufacturing. However, high cytotoxicity and… read more here.
Keywords: peptides polymer; gene delivery; based gene; polymer based ... See more keywords
Developing AAV-based gene therapy for adrenoleukodystrophy (X-ALD)
Sign Up to like & getrecommendations! Published in 2019 at "Cytotherapy"
DOI: 10.1016/j.jcyt.2019.04.046
Abstract: Background & Aim Adrenoleukodystrophy (X-ALD) is an X-linked disease. ALD is caused by mutations of the gene encoding ATP-Binding cassette subfamily D member 1 (ABCD1), a peroxisomal transmembrane protein. Dysfunctional ABCD1 protein results in excessive… read more here.
Keywords: therapy; ald; based gene; aav based ... See more keywords
Utilizing Epigenetic Modification as a Reactive Handle To Regulate RNA Function and CRISPR-Based Gene Regulation.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.3c01864
Abstract: The current methods to control RNA functions in living conditions are limited. The new RNA-controlling strategy presented in this study involves utilizing 5-formylcytidine (f5C)-directed base manipulation. This study shows that malononitrile and pyridine boranes can… read more here.
Keywords: based gene; crispr; rna; crispr based ... See more keywords
AlzGenPred - CatBoost-based gene classifier for predicting Alzheimer’s disease using high-throughput sequencing data
Sign Up to like & getrecommendations! Published in 2024 at "Scientific Reports"
DOI: 10.1038/s41598-024-82208-x
Abstract: AD is a progressive neurodegenerative disorder characterized by memory loss. Due to the advancement in next-generation sequencing, an enormous amount of AD-associated genomics data is available. However, the information about the involvement of these genes… read more here.
Keywords: catboost based; based gene; based features; alzgenpred catboost ... See more keywords
Implication of an exosome-based gene signature for estimating clinical outcomes of triple-negative breast cancer and assisting in individualized therapy
Sign Up to like & getrecommendations! Published in 2025 at "Scientific Reports"
DOI: 10.1038/s41598-025-16751-6
Abstract: Exosomes play a crucial role in triple-negative breast cancer (TNBC), influencing various aspects of tumor progression. Given the importance of exosomes in TNBC biology, we proposed a novel exosome-based model that was critically implicated in… read more here.
Keywords: exosome based; clinical outcomes; based gene; exosome ... See more keywords
A Comprehensive Review of Clinically Applied Adeno-Associated Virus-Based Gene Therapies for Ocular Disease.
Sign Up to like & getrecommendations! Published in 2025 at "Human gene therapy"
DOI: 10.1089/hum.2024.252
Abstract: The eye is an ideal target for gene therapy due its accessibility, immune privilege, small size, and compartmentalization. Adeno-associated virus (AAV) is the gold standard vector for gene delivery and can be injected via multiple… read more here.
Keywords: adeno associated; based gene; gene therapies; associated virus ... See more keywords
Benchmarking network-based gene prioritization methods for cerebral small vessel disease
Sign Up to like & getrecommendations! Published in 2021 at "Briefings in Bioinformatics"
DOI: 10.1093/bib/bbab006
Abstract: Abstract Network-based gene prioritization algorithms are designed to prioritize disease-associated genes based on known ones using biological networks of protein interactions, gene–disease associations (GDAs) and other relationships between biological entities. Various algorithms have been developed… read more here.
Keywords: network based; gene prioritization; disease; based gene ... See more keywords
Exploring automatic inconsistency detection for literature-based gene ontology annotation
Sign Up to like & getrecommendations! Published in 2022 at "Bioinformatics"
DOI: 10.1093/bioinformatics/btac230
Abstract: Abstract Motivation Literature-based gene ontology annotations (GOA) are biological database records that use controlled vocabulary to uniformly represent gene function information that is described in the primary literature. Assurance of the quality of GOA is… read more here.
Keywords: based gene; literature based; inconsistency; ontology ... See more keywords