Hands-on protocol for preparing water-soluble fractions from agri-food samples for NMR-based metabolomics analysis.
Sign Up to like & getrecommendations! Published in 2025 at "FEBS open bio"
DOI: 10.1002/2211-5463.70153
Abstract: The aim of this study was to address the lack of protocols for nuclear magnetic resonance (NMR)-based metabolomics in the agri-food sector by providing a reproducible workflow for the preparation and analysis of water-soluble metabolite… read more here.
Keywords: food; based metabolomics; analysis; agri food ... See more keywords
Exploring metabolic responses and pathway changes in CHO‐K1 cells under varied aeration conditions and copper supplementations using 1H NMR‐based metabolomics
Sign Up to like & getrecommendations! Published in 2024 at "Biotechnology Journal"
DOI: 10.1002/biot.202300495
Abstract: The optimization of bioprocess for CHO cell culture involves careful consideration of factors such as nutrient consumption, metabolic byproduct accumulation, cell growth, and monoclonal antibody (mAb) production. Valuable insights can be obtained by understanding cellular… read more here.
Keywords: based metabolomics; using nmr; culture; cell ... See more keywords
Chronic effects of copper in oysters Crassostrea hongkongensis under different exposure regimes as shown by NMR-based metabolomics.
Sign Up to like & getrecommendations! Published in 2017 at "Environmental toxicology and chemistry"
DOI: 10.1002/etc.3780
Abstract: Traditional metal toxicity tests on organisms have mainly focused on continuous exposure at a fixed concentration. However, organisms are more likely exposed to pollutants intermittently in estuarine environments that are significantly impacted by anthropogenic activity.… read more here.
Keywords: crassostrea hongkongensis; exposure; copper; exposure regimes ... See more keywords
Potential of in vitro NMR of bio-fluids and tissues in clinical research.
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DOI: 10.1002/nbm.4686
Abstract: Body-fluids, cells and tissues contain a wide variety of metabolites that consist of a mixture of various low-molecular-weight compounds that includes amino acids/peptides, lipids, nucleic acids, and organic acids, which makes the comprehensive analysis more… read more here.
Keywords: vitro nmr; nmr based; based metabolomics; nmr ... See more keywords
Investigation on the immunotoxicity induced by Emamectin benzoate on THP‐1 macrophages based on metabolomics analysis
Sign Up to like & getrecommendations! Published in 2023 at "Environmental Toxicology"
DOI: 10.1002/tox.23747
Abstract: Emamectin benzoate (EMB) is an insecticide extensively used in agricultural area. Assessing the toxic effects of EMB in mammals or humans and its endogenous metabolites alteration are the appropriate means of evaluating its risks to… read more here.
Keywords: macrophages based; immunotoxicity; based metabolomics; analysis ... See more keywords
Using the IDEOM Workflow for LCMS-Based Metabolomics Studies of Drug Mechanisms.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0239-3_21
Abstract: Rapid advancements in metabolomics technologies have allowed for application of liquid chromatography mass spectrometry (LCMS)-based metabolomics to investigate a wide range of biological questions. In addition to an important role in studies of cellular biochemistry… read more here.
Keywords: lcms based; lcms; drug; based metabolomics ... See more keywords
LC-MS/MS-Based Metabolomics for Cell Cultures.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9477-9_10
Abstract: Metabolomics, a comprehensive analysis of metabolites in biological specimens (e.g., cells, body fluids, tissues, exhaled air, plants), offers promising tools in health, nutrition, biotechnology, and food sciences. Here we describe methods of LC-MS/MS-based analyses for… read more here.
Keywords: analysis; biology; metabolomics cell; based metabolomics ... See more keywords
NMR-Based Metabolomics in Gallbladder Cancer Research.
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DOI: 10.1007/978-1-4939-9690-2_14
Abstract: Gallbladder cancer (GBC) is a common form of malignancy, which has high incidence rates in the northern parts of India, South America, Japan, and in Native American populations. A few metabolomic studies have revealed changes… read more here.
Keywords: metabolomics gallbladder; cancer research; based metabolomics; cancer ... See more keywords
Biomarker Discovery Using NMR-Based Metabolomics of Tissue.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9690-2_15
Abstract: NMR-based metabolomics has shown promise in the diagnosis of diseases as it enables identification and quantification of metabolic biomarkers. Using high-resolution magic-angle-spinning (HR-MAS) NMR spectroscopy, metabolic profiles from intact tissue specimens can be obtained with… read more here.
Keywords: biomarker discovery; tissue; metabolomics tissue; based metabolomics ... See more keywords
NMR-Based Metabolomics in Cancer Research.
Sign Up to like & getrecommendations! Published in 2021 at "Advances in experimental medicine and biology"
DOI: 10.1007/978-3-030-51652-9_14
Abstract: Nuclear magnetic resonance (NMR) spectroscopy offers reproducible quantitative analysis and structural identification of metabolites in various complex biological samples, such as biofluids (plasma, serum, and urine), cells, tissue extracts, and even intact organs. Therefore, NMR-based… read more here.
Keywords: metabolomics cancer; cancer research; nmr based; based metabolomics ... See more keywords
Mass Spectrometry-based Metabolomics in Translational Research.
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DOI: 10.1007/978-981-33-6064-8_19
Abstract: Metabolomics is the systematic study of metabolite profiles of complex biological systems, and involves the systematic identification and quantification of metabolites. Metabolism is integrated with all biochemical reactions in biological systems; thus metabolite profiles provide… read more here.
Keywords: research; mass spectrometry; based metabolomics; spectrometry based ... See more keywords