Displacement chemistry-based nanopore analysis of nucleic acids in complicated matrices.
Sign Up to like & getrecommendations! Published in 2018 at "Chemical communications"
DOI: 10.1039/c8cc07944g
Abstract: To overcome the effect of other components of complicated biological samples on nanopore stochastic sensing, displacement chemical reaction was utilized to selectively extract the target nucleic acid from whole blood. Given its simplicity and high… read more here.
Keywords: nucleic acids; chemistry; based nanopore; nanopore analysis ... See more keywords
Building a nomogram plot based on the nanopore targeted sequencing for predicting urinary tract pathogens and differentiating from colonizing bacteria
Sign Up to like & getrecommendations! Published in 2023 at "Frontiers in Cellular and Infection Microbiology"
DOI: 10.3389/fcimb.2023.1142426
Abstract: Background The identification of uropathogens (UPBs) and urinary tract colonizing bacteria (UCB) conduces to guide the antimicrobial therapy to reduce resistant bacterial strains and study urinary microbiota. This study established a nomogram based on the… read more here.
Keywords: urinary tract; tract; colonizing bacteria; nanopore targeted ... See more keywords
Rapid PCR-Based Nanopore Adaptive Sequencing Improves Sensitivity and Timeliness of Viral Clinical Detection and Genome Surveillance
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2022.929241
Abstract: Nanopore sequencing has been widely used for the real-time detection and surveillance of pathogens with portable MinION. Nanopore adaptive sequencing can enrich on-target sequences without additional pretreatment. In this study, the performance of adaptive sequencing… read more here.
Keywords: sensitivity timeliness; surveillance; adaptive sequencing; nanopore adaptive ... See more keywords