Mass Spectrometry-Based Proteomics.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7804-5_9
Abstract: Proteomics is the large-scale analysis of proteins rendered possible by modern mass spectrometry analysis methods capable of identifying thousands of peptides/proteins in a fast high-throughput manner. Here I describe protocols for the preparation of fungal… read more here.
Keywords: spectrometry; mass spectrometry; analysis; based proteomics ... See more keywords
A Roadmap to Domain Based Proteomics.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8736-8_16
Abstract: Protein domains are reusable segments of proteins and play an important role in protein evolution. By combining the elements from a relatively small set of domains into unique arrangements, a large number of distinct proteins can… read more here.
Keywords: protein; based proteomics; biology; roadmap domain ... See more keywords
Application of an Aptamer-Based Proteomics Assay (SOMAscan™) in Rat Cerebrospinal Fluid.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9706-0_13
Abstract: The exploration of the cerebrospinal fluid (CSF) through proteomics techniques might help in the search of molecular biomarkers relevant to neurological pathologies. Aiming this, we describe here a commercially available multiplexed proteomics technology based on… read more here.
Keywords: aptamer based; based proteomics; application aptamer; rat ... See more keywords
Combinatorial Electrophoresis and Mass Spectrometry-Based Proteomics in Breast Milk for Breast Cancer Biomarker Discovery.
Sign Up to like & getrecommendations! Published in 2019 at "Advances in experimental medicine and biology"
DOI: 10.1007/978-3-030-15950-4_26
Abstract: Innovations in approaches for early detection and individual risk assessment of different cancers, including breast cancer (BC), are needed to reduce cancer morbidity and associated mortality. The assessment of potential cancer biomarkers in accessible bodily… read more here.
Keywords: milk; mass spectrometry; based proteomics; breast cancer ... See more keywords
Liquid chromatography mass spectrometry-based proteomics of Escherichia coli single colony
Sign Up to like & getrecommendations! Published in 2021 at "MethodsX"
DOI: 10.1016/j.mex.2021.101277
Abstract: The Escherichia coli proteome is the most extensively characterized and studied of all prokaryotic proteomes. Despite this, large scale bacterial proteomics experiments performed on E. coli cells grown in liquid cultures have failed to identify… read more here.
Keywords: colony; mass spectrometry; escherichia coli; based proteomics ... See more keywords
Biodegradation of Punicalagin into Ellagic Acid by Selected Probiotic Bacteria: A Study of the Underlying Mechanisms by MS-Based Proteomics
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Agricultural and Food Chemistry"
DOI: 10.1021/acs.jafc.2c06585
Abstract: Pomegranate (Punica granatum L.) is a well-known source of bioactive phenolic compounds such as ellagitannins, anthocyanins, and flavanols. Punicalagin, one of the main constituents of pomegranate, needs to be biodegraded by bacteria to yield metabolites… read more here.
Keywords: punicalagin ellagic; biodegradation punicalagin; based proteomics; ellagic acid ... See more keywords
Evaluation and optimization of reduction and alkylation methods to maximize peptide identification with MS-based proteomics.
Sign Up to like & getrecommendations! Published in 2017 at "Molecular bioSystems"
DOI: 10.1039/c7mb00393e
Abstract: Mass spectrometry (MS) has become an increasingly important technique to analyze proteins. In popular bottom-up MS-based proteomics, reduction and alkylation are routine steps to facilitate peptide identification. However, incomplete reactions and side reactions may occur,… read more here.
Keywords: reduction; based proteomics; reduction alkylation; alkylation ... See more keywords
The PRIDE database resources in 2022: a hub for mass spectrometry-based proteomics evidences.
Sign Up to like & getrecommendations! Published in 2021 at "Nucleic acids research"
DOI: 10.1093/nar/gkab1038
Abstract: The PRoteomics IDEntifications (PRIDE) database (https://www.ebi.ac.uk/pride/) is the world's largest data repository of mass spectrometry-based proteomics data. PRIDE is one of the founding members of the global ProteomeXchange (PX) consortium and an ELIXIR core data… read more here.
Keywords: pride; mass spectrometry; pride database; mass ... See more keywords
Based on proteomics to explore the mechanism of mecobalamin promoting the repair of injured peripheral nerves.
Sign Up to like & getrecommendations! Published in 2022 at "Canadian journal of physiology and pharmacology"
DOI: 10.1139/cjpp-2021-0692
Abstract: Mecobalamin is commonly used in the adjuvant intervention of various peripheral nerve injuries. Actin cytoskeleton plays a role in regeneration of myelin and axon. Therefore, the purpose of this study was to explore the possibility… read more here.
Keywords: actin cytoskeleton; based proteomics; group; proteomics explore ... See more keywords
iTRAQ-Based Proteomics Analysis Reveals the Effect of Rhubarb in Rats with Ischemic Stroke
Sign Up to like & getrecommendations! Published in 2018 at "BioMed Research International"
DOI: 10.1155/2018/6920213
Abstract: Background. Rhubarb, a traditional Chinese medicine, promotes viscera and remove blood stasis. Rhubarb is skilled in smoothening meridians, improving blood circulation which exhibits better efficacy on cerebral ischemic stroke. In this study, we aimed to… read more here.
Keywords: rhubarb; ischemic stroke; analysis; based proteomics ... See more keywords
Abstract 3974: Discovery and validation of therapeutic targets in immune cells by mass spectrometry-based proteomics
Sign Up to like & getrecommendations! Published in 2023 at "Cancer Research"
DOI: 10.1158/1538-7445.am2023-3974
Abstract: Immuno-oncology (IO) has substantially improved the survival of cancer patients over the past several years encouraging the discovery of novel IO targets which are typically proteins expressed on the surface of immune cells. Sensitive quantification… read more here.
Keywords: immune cells; discovery validation; based proteomics; mass spectrometry ... See more keywords