Metabolic phenotyping of CHO cells varying in cellular biomass accumulation and maintenance during fed‐batch culture
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.26485
Abstract: CHO cell lines capable of high‐level recombinant protein product biosynthesis during fed‐batch culture are still generally obtained by intensive empirical screening of transfected cells rather than knowledge‐guided cellular engineering. In this study, we investigate how… read more here.
Keywords: batch culture; fed batch; cho; cho cell ... See more keywords
Development of a chemically defined platform fed-batch culture media for monoclonal antibody-producing CHO cell lines with optimized choline content
Sign Up to like & getrecommendations! Published in 2017 at "Cytotechnology"
DOI: 10.1007/s10616-017-0185-1
Abstract: A chemically defined platform basal medium and feed media were developed using a single Chinese hamster ovary (CHO) cell line that produces a monoclonal antibody (mAb). Cell line A, which showed a peak viable cell… read more here.
Keywords: medium; platform; batch culture; fed batch ... See more keywords
Estimation of plasmid concentration in batch culture of Escherichia coli DH5α via simple state observer
Sign Up to like & getrecommendations! Published in 2018 at "Chemical Papers"
DOI: 10.1007/s11696-018-0478-7
Abstract: The aim of this paper is to present an alternative state observer structure for online estimation purposes of the key dynamical variables in a class of batch culture for plasmid production; the latter has been… read more here.
Keywords: methodology; estimation; batch culture; concentration ... See more keywords
A Novel Fractional Microbial Batch Culture Process and Parameter Identification
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DOI: 10.1007/s12591-017-0381-7
Abstract: This paper considers the microbial batch culture for producing 1,3-propanediol(1,3-PD) via glycerol disproportionation. Due to the nature of the fractional order operations, a novel fractional order model, which is based upon the original ordinary differential… read more here.
Keywords: parameter identification; novel fractional; batch culture; microbial batch ... See more keywords
Estimating gas volume from headspace pressure in a batch culture system
Sign Up to like & getrecommendations! Published in 2018 at "Canadian Journal of Animal Science"
DOI: 10.1139/cjas-2017-0100
Abstract: Abstract: Two in vitro batch culture experiments were conducted to obtain a total of 1811 paired data for headspace gas pressure (GP; PSI) and volume (GV; mL). Data were used to generate and evaluate an… read more here.
Keywords: gas; headspace; batch culture; batch ... See more keywords
Amounts and species of probiotic lactic acid bacteria affect stimulation of short-chain fatty acid production in fecal batch culture
Sign Up to like & getrecommendations! Published in 2022 at "Bioscience of Microbiota, Food and Health"
DOI: 10.12938/bmfh.2022-048
Abstract: The effects of lactate and probiotic lactic acid bacteria (LAB) on intestinal fermentation were analyzed using a fecal batch culture. Lactate was efficiently metabolized to butyrate and propionate by butyrate-utilizing bacteria in fecal fermentation. Probiotic… read more here.
Keywords: fecal batch; probiotic lactic; acid bacteria; lactic acid ... See more keywords