Protein quantification by bicinchoninic acid (BCA) assay follows complex kinetics and can be performed at short incubation times.
Sign Up to like & getrecommendations! Published in 2020 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2020.113904
Abstract: Amongst the available methodologies for protein determination, the bicinchoninic acid (BCA) assay highlights for its simplicity, sensitivity, repeatability and reproducibility. Nevertheless, in spite that the general principle behind this methodology is known, there are still… read more here.
Keywords: acid bca; incubation; incubation times; bicinchoninic acid ... See more keywords
Crystal violet staining is a reliable alternative to bicinchoninic acid assay-based normalization.
Sign Up to like & getrecommendations! Published in 2022 at "BioTechniques"
DOI: 10.2144/btn-2022-0064
Abstract: Experimental data with cells often require normalization. The frequently used bicinchoninic acid (BCA) assay, in fact, indicates protein content but is influenced by incubation time, pH etc. A simple, rapid and reliable alternative is desirable.… read more here.
Keywords: violet staining; violet; bicinchoninic acid; normalization ... See more keywords