A bicistronic vector with destabilized mRNA secondary structure yields scalable higher titer expression of human neurturin in E. coli
Sign Up to like & getrecommendations! Published in 2017 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.26299
Abstract: Human neurturin (NTN) is a cystine knot growth factor with potential therapeutic use in diseases such as Parkinson's and diabetes. Scalable high titer production of native NTN is particularly challenging because of the cystine knot… read more here.
Keywords: bicistronic vector; secondary structure; mrna secondary; expression ... See more keywords
Co-delivery of genes can be confounded by bicistronic vector design
Sign Up to like & getrecommendations! Published in 2022 at "Mrs Communications"
DOI: 10.1557/s43579-021-00128-7
Abstract: Maximizing the efficiency of nanocarrier-mediated co-delivery of genes for co-expression in the same cell is critical for many applications. Strategies to maximize co-delivery of nucleic acids (NA) focused largely on carrier systems, with little attention… read more here.
Keywords: genes confounded; confounded bicistronic; design; delivery genes ... See more keywords