Bimolecular Fluorescence Complementation to Visualize Protein-Protein Interactions in Cells.
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DOI: 10.1007/978-1-0716-2051-9_5
Abstract: Examining protein-protein interactions provides critical insight into numerous human diseases and infections. Here we describe a protocol for bimolecular fluorescence complementation, which can be used to directly visualize and characterize intracellular protein-protein interactions and ascertain… read more here.
Keywords: protein interactions; protein protein; fluorescence complementation; protein ... See more keywords
Quantification of Protein Aggregates Using Bimolecular Fluorescence Complementation.
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DOI: 10.1007/978-1-4939-8820-4_11
Abstract: Neuronal accumulations of the presynaptic protein α-synuclein represent a hallmark of Parkinson's disease (PD). Multiple system atrophy and dementia with Lewy bodies are other neurodegenerative diseases in which α-synuclein aggregates are the main pathological characteristic.… read more here.
Keywords: protein; fluorescence complementation; aggregation; bimolecular fluorescence ... See more keywords
A novel orange-colored bimolecular fluorescence complementation (BiFC) assay using monomeric Kusabira-Orange protein.
Sign Up to like & getrecommendations! Published in 2018 at "BioTechniques"
DOI: 10.2144/btn-2017-0121
Abstract: The bimolecular fluorescence complementation (BiFC) assay was developed as a tool for the visualization of protein-protein interactions in living cells. To date, many types of BiFC systems with distinct colors have been developed. Most of… read more here.
Keywords: protein; bifc; complementation; orange ... See more keywords