Improving Structural Stability and Anticoagulant Activity of a Thrombin Binding Aptamer by Aromatic Modifications
Sign Up to like & getrecommendations! Published in 2022 at "ChemBioChem"
DOI: 10.1002/cbic.202100670
Abstract: The thrombin binding aptamer (TBA) is a 15‐mer DNA oligonucleotide (5′‐GGT TGG TGT GGT TGG‐3′), that can form a stable intramolecular antiparallel chair‐like G‐quadruplex structure. This aptamer shows anticoagulant properties by interacting with one of… read more here.
Keywords: thrombin binding; stability; anticoagulant activity; binding aptamer ... See more keywords
The "Janus face" of the thrombin binding aptamer: Investigating the anticoagulant and antiproliferative properties through straightforward chemical modifications.
Sign Up to like & getrecommendations! Published in 2018 at "Bioorganic chemistry"
DOI: 10.1016/j.bioorg.2017.11.005
Abstract: BACKGROUND The thrombin binding aptamer (TBA) is endowed with both anticoagulant and antiproliferative activities. Its chemico-physical and/or biological properties can be tuned by the site-specific replacement of selected residues. METHODS Four oligodeoxynucleotides (ODNs) based on… read more here.
Keywords: binding aptamer; anticoagulant antiproliferative; antiproliferative properties; residues positions ... See more keywords
A bivalent binding aptamer-cDNA on MoS2 nanosheets based fluorescent aptasensor for detection of aflatoxin M1.
Sign Up to like & getrecommendations! Published in 2021 at "Talanta"
DOI: 10.1016/j.talanta.2021.122779
Abstract: To ensure the safety of dairy products, especially milk, and consequently protect human health, accurate and simple analytical techniques are highly necessary to determine the low concentration of aflatoxin M1 (AFM1) as an important carcinogen.… read more here.
Keywords: detection; bivalent binding; binding aptamer; fluorescent aptasensor ... See more keywords
The Arsenic Binding Aptamer Cannot Bind Arsenic: Critical Evaluation of Aptamer Selection and Binding.
Sign Up to like & getrecommendations! Published in 2019 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.9b02789
Abstract: An arsenic binding aptamer named Ars-3 was reported in 2009, and it has been used for detection of As(III) in more than two dozen papers. In this work, we performed extensive binding assays using isothermal… read more here.
Keywords: binding aptamer; selection; aptamer bind; arsenic binding ... See more keywords
DNA-Crosslinked 2D Photonic Crystal Hydrogels for Detection of Adenosine Actuated by an Adenosine-Binding Aptamer.
Sign Up to like & getrecommendations! Published in 2022 at "ACS sensors"
DOI: 10.1021/acssensors.1c02424
Abstract: There is a need to develop versatile sensing motifs that can be used to detect a variety of chemical targets in resource-limited settings, for example, at the point of care. While numerous sensing technologies have… read more here.
Keywords: dna crosslinked; photonic crystal; binding aptamer; adenosine binding ... See more keywords
Inhibition of thrombin activity by a covalent-binding aptamer and reversal by the complementary strand antidote.
Sign Up to like & getrecommendations! Published in 2021 at "Chemical communications"
DOI: 10.1039/d0cc08109d
Abstract: Alleviating the potential risk of irreversible adverse drug effects has been an important and challenging issue for the development of covalent drugs. Here we created a DNA-aptamer-type covalent drug by introducing a sulfonyl fluoride warhead… read more here.
Keywords: binding aptamer; strand antidote; covalent; complementary strand ... See more keywords
Unlike RNA-TBA (rTBA), iso-rTBA, the 2'-5'-linked RNA-thrombin-binding aptamer, is a functional equivalent of TBA.
Sign Up to like & getrecommendations! Published in 2023 at "Chemical communications"
DOI: 10.1039/d2cc05718b
Abstract: An antiparallel, functional RNA G-quadruplex of the 2'-5'-linked thrombin-binding aptamer (iso-rTBA) is reported for the first time. It can inhibit clotting and is remarkably stable to nuclease-degradation, besides having high thermal stability. It is thus,… read more here.
Keywords: tba; binding aptamer; tba rtba; rtba ... See more keywords
Binding mode of a cationic porphyrin to parallel and antiparallel thrombin binding aptamer G-quadruplex
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Biomolecular Structure and Dynamics"
DOI: 10.1080/07391102.2019.1642241
Abstract: Abstract The spectral properties of meso-tetrakis (N-methylpyridinium-4-yl)porphyrin (TMPyP) in the presence of parallel and antiparallel G-quadruplexes formed from a thrombin-binding aptamer G-quadruplex (5′-G3T2G3TGTG3T2G3) were investigated in this study. Red shift and hypochromism in the Soret… read more here.
Keywords: binding aptamer; antiparallel quadruplexes; porphyrin; parallel antiparallel ... See more keywords
Structural properties and anticoagulant/cytotoxic activities of heterochiral enantiomeric thrombin binding aptamer (TBA) derivatives
Sign Up to like & getrecommendations! Published in 2020 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkaa1109
Abstract: Abstract The thrombin binding aptamer (TBA) possesses promising antiproliferative properties. However, its development as an anticancer agent is drastically impaired by its concomitant anticoagulant activity. Therefore, suitable chemical modifications in the TBA sequence would be… read more here.
Keywords: aptamer tba; tba; tba derivatives; binding aptamer ... See more keywords
The Characterization of Binding between Aptamer and Bisphenol A and Developing Electrochemical Aptasensors for Bisphenol A with Rationally Engineered Aptamers
Sign Up to like & getrecommendations! Published in 2022 at "Biosensors"
DOI: 10.3390/bios12110913
Abstract: Bisphenol A (BPA) is widely used in the manufacture of polycarbonate and epoxy-resin-based products, and BPA contamination often happens in a variety of types of environment and food stuffs. BPA can cause many harmful effects… read more here.
Keywords: binding aptamer; bpa; detection; bisphenol ... See more keywords
Alpha-Deoxyguanosine to Reshape the Alpha-Thrombin Binding Aptamer
Sign Up to like & getrecommendations! Published in 2023 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms24098406
Abstract: Modification of DNA aptamers is aimed at increasing their thermodynamic stability, and improving affinity and resistance to biodegradation. G-quadruplex DNA aptamers are a family of affinity ligands that form non-canonical DNA assemblies based on a… read more here.
Keywords: alpha deoxyguanosine; binding aptamer; alpha thrombin; thrombin binding ... See more keywords