A Ligand-Binding Assay to Measure the Affinity and Specificity of Sterol-Binding Proteins In Vitro.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7183-1_25
Abstract: Sterols are major constituents of the plasma membrane of eukaryotic cells and serve as a precursor for several classes of signaling molecules, including steroids and hydroxy sterols. They maintain the functionality and permeability barrier of… read more here.
Keywords: binding assay; specificity sterol; assay measure; measure affinity ... See more keywords
Non-radioactive binding assay for bradykinin and angiotensin receptors.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in cell biology"
DOI: 10.1016/bs.mcb.2018.08.002
Abstract: G protein-coupled receptors (GPCRs) are the largest family of membrane protein playing an important role in cellular signal transduction. GPCRs interact with different molecules acting as ligands capable to trigger responses on signaling pathway. Those… read more here.
Keywords: bradykinin angiotensin; binding assay; assay bradykinin; angiotensin receptors ... See more keywords
A plate-based histo-blood group antigen binding assay for evaluation of human norovirus receptor binding ability.
Sign Up to like & getrecommendations! Published in 2017 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2017.06.012
Abstract: Human norovirus is a leading cause of gastroenteritis worldwide. Although two in vitro cultivation methods have been reported, they cannot provide mechanistic insights into viral inactivation. Receptor-binding assays supplement these assays and give insight into capsid… read more here.
Keywords: plate based; binding assay; receptor binding; human norovirus ... See more keywords
Biolayer Interferometry-based FcγRIIa binding assay for a therapeutic antibody with strong effector function.
Sign Up to like & getrecommendations! Published in 2020 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2020.113842
Abstract: FcγRIIa receptor binding is part of the mechanism of action for many therapeutic antibodies. AlphaScreen® technology and Biolayer Interferometry (BLI) are often used to assess protein-protein interactions. Recently we demonstrated that the presence of aggregates… read more here.
Keywords: based riia; riia; binding assay; biolayer interferometry ... See more keywords
Miniaturization of a transthyretin binding assay using a fluorescent probe for high throughput screening of thyroid hormone disruption in environmental samples.
Sign Up to like & getrecommendations! Published in 2017 at "Chemosphere"
DOI: 10.1016/j.chemosphere.2016.12.119
Abstract: Thyroid hormone (TH) disrupting compounds are potentially important environmental contaminants due to their possible adverse neurological and developmental effects on both humans and wildlife. Currently, the most successful bio-analytical method to detect and evaluate TH… read more here.
Keywords: binding assay; environmental samples; thyroid hormone; high throughput ... See more keywords
Competitive binding assays for measuring the binding affinity of thyroid-disrupting chemicals for integrin αvβ3.
Sign Up to like & getrecommendations! Published in 2020 at "Chemosphere"
DOI: 10.1016/j.chemosphere.2020.126034
Abstract: Some thyroid-disrupting chemicals (TDCs) affect thyroid function by activating the pathways mediated by a typical thyroid hormone (TH) membrane receptor, integrin αvβ3. The present study introduces improved competitive binding assays for the rapid and sensitive… read more here.
Keywords: binding assay; radioligand competitive; binding; competitive binding ... See more keywords
Creation of Fluorescent RXR Antagonists Based on CBTF-EE and Application to a Fluorescence Polarization Binding Assay.
Sign Up to like & getrecommendations! Published in 2021 at "ACS medicinal chemistry letters"
DOI: 10.1021/acsmedchemlett.1c00201
Abstract: Retinoid X receptor (RXR) ligands often bind in modes in which the carboxy group forms a hydrogen bond inside the ligand-binding pocket (LBP). However, our previously reported RXR antagonist, CBTF-EE (4a), binds with its carboxy… read more here.
Keywords: binding assay; fluorescence polarization; fluorescence; rxr antagonists ... See more keywords
C3d-binding assay for the detection of complement activating HLA antibodies: A useful tool for allocation to highly sensitised recipients in the post-CDC era?
Sign Up to like & getrecommendations! Published in 2023 at "HLA"
DOI: 10.1111/tan.15010
Abstract: INTRODUCTION The CDC crossmatch test is being phased out in solid organ donor allocation, and standard luminex single antigen bead assays do not differentiate complement activating function of HLA antibodies. The current study investigated the… read more here.
Keywords: hla antibodies; highly sensitised; complement activating; cdc ... See more keywords
Surface plasmon resonance as a tool for ligand-binding assay reagent characterization in bioanalysis of biotherapeutics.
Sign Up to like & getrecommendations! Published in 2018 at "Bioanalysis"
DOI: 10.4155/bio-2017-0271
Abstract: Ligand-binding assay (LBA) performance depends on quality reagents. Strategic reagent screening and characterization is critical to LBA development, optimization and validation. Application of advanced technologies expedites the reagent screening and assay development process. By evaluating… read more here.
Keywords: binding assay; ligand binding; surface plasmon; plasmon resonance ... See more keywords
The role of ligand-binding assay and LC-MS in the bioanalysis of complex protein and oligonucleotide therapeutics.
Sign Up to like & getrecommendations! Published in 2021 at "Bioanalysis"
DOI: 10.4155/bio-2021-0009
Abstract: Ligand-binding assay (LBA) and LC-MS have been the preferred bioanalytical techniques for the quantitation and biotransformation assessment of various therapeutic modalities. This review provides an overview of the applications of LBA, LC-MS/MS and LC-HRMS for… read more here.
Keywords: binding assay; bioanalysis; oligonucleotide therapeutics; complex protein ... See more keywords