A Ligand-Binding Assay to Measure the Affinity and Specificity of Sterol-Binding Proteins In Vitro.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7183-1_25
Abstract: Sterols are major constituents of the plasma membrane of eukaryotic cells and serve as a precursor for several classes of signaling molecules, including steroids and hydroxy sterols. They maintain the functionality and permeability barrier of… read more here.
Keywords: binding assay; specificity sterol; assay measure; measure affinity ... See more keywords
Non-radioactive binding assay for bradykinin and angiotensin receptors.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in cell biology"
DOI: 10.1016/bs.mcb.2018.08.002
Abstract: G protein-coupled receptors (GPCRs) are the largest family of membrane protein playing an important role in cellular signal transduction. GPCRs interact with different molecules acting as ligands capable to trigger responses on signaling pathway. Those… read more here.
Keywords: bradykinin angiotensin; binding assay; assay bradykinin; angiotensin receptors ... See more keywords
A plate-based histo-blood group antigen binding assay for evaluation of human norovirus receptor binding ability.
Sign Up to like & getrecommendations! Published in 2017 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2017.06.012
Abstract: Human norovirus is a leading cause of gastroenteritis worldwide. Although two in vitro cultivation methods have been reported, they cannot provide mechanistic insights into viral inactivation. Receptor-binding assays supplement these assays and give insight into capsid… read more here.
Keywords: plate based; binding assay; receptor binding; human norovirus ... See more keywords
Biolayer Interferometry-based FcγRIIa binding assay for a therapeutic antibody with strong effector function.
Sign Up to like & getrecommendations! Published in 2020 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2020.113842
Abstract: FcγRIIa receptor binding is part of the mechanism of action for many therapeutic antibodies. AlphaScreen® technology and Biolayer Interferometry (BLI) are often used to assess protein-protein interactions. Recently we demonstrated that the presence of aggregates… read more here.
Keywords: based riia; riia; binding assay; biolayer interferometry ... See more keywords
Miniaturization of a transthyretin binding assay using a fluorescent probe for high throughput screening of thyroid hormone disruption in environmental samples.
Sign Up to like & getrecommendations! Published in 2017 at "Chemosphere"
DOI: 10.1016/j.chemosphere.2016.12.119
Abstract: Thyroid hormone (TH) disrupting compounds are potentially important environmental contaminants due to their possible adverse neurological and developmental effects on both humans and wildlife. Currently, the most successful bio-analytical method to detect and evaluate TH… read more here.
Keywords: binding assay; environmental samples; thyroid hormone; high throughput ... See more keywords
Competitive binding assays for measuring the binding affinity of thyroid-disrupting chemicals for integrin αvβ3.
Sign Up to like & getrecommendations! Published in 2020 at "Chemosphere"
DOI: 10.1016/j.chemosphere.2020.126034
Abstract: Some thyroid-disrupting chemicals (TDCs) affect thyroid function by activating the pathways mediated by a typical thyroid hormone (TH) membrane receptor, integrin αvβ3. The present study introduces improved competitive binding assays for the rapid and sensitive… read more here.
Keywords: binding assay; radioligand competitive; binding; competitive binding ... See more keywords
Creation of Fluorescent RXR Antagonists Based on CBTF-EE and Application to a Fluorescence Polarization Binding Assay.
Sign Up to like & getrecommendations! Published in 2021 at "ACS medicinal chemistry letters"
DOI: 10.1021/acsmedchemlett.1c00201
Abstract: Retinoid X receptor (RXR) ligands often bind in modes in which the carboxy group forms a hydrogen bond inside the ligand-binding pocket (LBP). However, our previously reported RXR antagonist, CBTF-EE (4a), binds with its carboxy… read more here.
Keywords: binding assay; fluorescence polarization; fluorescence; rxr antagonists ... See more keywords
Novel PPAR-γ agonists as potential neuroprotective agents against Alzheimer's disease: rational design, synthesis, in silico evaluation, PPAR-γ binding assay and transactivation and expression studies
Sign Up to like & getrecommendations! Published in 2024 at "RSC Advances"
DOI: 10.1039/d4ra06330a
Abstract: Alzheimer's disease (AD) is a neurological disorder. It is caused by accumulation of amyloid beta (Aβ) plaques and tau tangles, which gradually leads to cognitive decline and memory loss. Peroxisome proliferator-activated receptor gamma (PPAR-γ), a… read more here.
Keywords: alzheimer disease; ppar agonists; ppar; binding assay ... See more keywords
Ratio of Von Willebrand Collagen Binding Assay and Von Willebrand Antigen Can Predict Multimer Size in Von Willebrand Disease
Sign Up to like & getrecommendations! Published in 2025 at "Haemophilia"
DOI: 10.1111/hae.70025
Abstract: Von Willebrand disease (vWD) is a common bleeding disorder with different subtypes. Laboratory diagnosis is challenging, involving several expensive and complex assays. The von Willebrand factor (vWF) collagen binding assay (VWF:CB) has been described to… read more here.
Keywords: binding assay; willebrand; willebrand disease; collagen binding ... See more keywords
C3d-binding assay for the detection of complement activating HLA antibodies: A useful tool for allocation to highly sensitised recipients in the post-CDC era?
Sign Up to like & getrecommendations! Published in 2023 at "HLA"
DOI: 10.1111/tan.15010
Abstract: INTRODUCTION The CDC crossmatch test is being phased out in solid organ donor allocation, and standard luminex single antigen bead assays do not differentiate complement activating function of HLA antibodies. The current study investigated the… read more here.
Keywords: hla antibodies; highly sensitised; complement activating; cdc ... See more keywords
Experimental Conditions to Retrieve Intrinsic Cooperativity α Directly from Single Binding Assay Data Exemplified by the Ternary Complex Formation of FKBP12, MAPRE1 and Macrocyclic Molecular Glues
Sign Up to like & getrecommendations! Published in 2025 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms26072936
Abstract: The incorporation of disease-relevant targets in a compound-dependent manner into ternary complexes with an assisting chaperone protein has become a common modality. Among the various types of ternary complex-forming compounds, molecular glues are of particular… read more here.
Keywords: molecular glues; binding assay; cooperativity; binding protein ... See more keywords