Identification of Pyrrolidine‐3‐acetic Acid Derived Oximes as Potent Inhibitors of γ‐Aminobutyric Acid Transporter 1 through Library Screening with MS Binding Assays
Sign Up to like & getrecommendations! Published in 2018 at "ChemMedChem"
DOI: 10.1002/cmdc.201800556
Abstract: In this study, pyrrolidine‐3‐acetic acid derived oxime libraries were applied to the concept of library screening by MS Binding Assays, as a powerful technique to reveal new potent murine γ‐aminobutyric acid transporter subtype (mGAT1) inhibitors.… read more here.
Keywords: acid derived; pyrrolidine acetic; binding assays; acetic acid ... See more keywords
Benocyclidine (BTCP) as Non‐labelled Reporter Ligand for MS Binding Assays for the PCP Ion Channel Binding Site of the Desensitized Torpedo Nicotinic Acetylcholine Receptor (nAChR)
Sign Up to like & getrecommendations! Published in 2023 at "ChemMedChem"
DOI: 10.1002/cmdc.202300048
Abstract: In this study we present MS Binding Assays for the PCP ion channel binding site of Torpedo californica nicotinic acetylcholine receptor (nAChR) as an alternative to radioligand binding assays. As MS Marker Benocyclidine (BTCP) was… read more here.
Keywords: pcp ion; ion channel; channel binding; binding site ... See more keywords
Competitive binding assays for measuring the binding affinity of thyroid-disrupting chemicals for integrin αvβ3.
Sign Up to like & getrecommendations! Published in 2020 at "Chemosphere"
DOI: 10.1016/j.chemosphere.2020.126034
Abstract: Some thyroid-disrupting chemicals (TDCs) affect thyroid function by activating the pathways mediated by a typical thyroid hormone (TH) membrane receptor, integrin αvβ3. The present study introduces improved competitive binding assays for the rapid and sensitive… read more here.
Keywords: binding assay; radioligand competitive; binding; competitive binding ... See more keywords
Behavior of ligand binding assays with crowded surfaces: Molecular model of antigen capture by antibody-conjugated nanoparticles
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DOI: 10.1371/journal.pone.0185518
Abstract: Ligand-receptor binding is of utmost importance in several biologically related disciplines. Ligand binding assays (LBA) use the high specificity and high affinity of ligands to detect, target or measure a specific receptors. One particular example… read more here.
Keywords: antibody conjugated; antibody; ligand binding; conjugated nanoparticles ... See more keywords
Fluorescence based HTS-compatible ligand binding assays for dopamine D3 receptors in baculovirus preparations and live cells
Sign Up to like & getrecommendations! Published in 2023 at "Frontiers in Molecular Biosciences"
DOI: 10.3389/fmolb.2023.1119157
Abstract: Dopamine receptors are G-protein-coupled receptors that are connected to severe neurological disorders. The development of new ligands targeting these receptors enables gaining a deeper insight into the receptor functioning, including binding mechanisms, kinetics and oligomerization.… read more here.
Keywords: ligand binding; dopamine receptors; microscopy; ligand ... See more keywords
Surfactant-Assisted Label-Free Fluorescent Aptamer Biosensors and Binding Assays
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DOI: 10.3390/bios13040434
Abstract: Using DNA staining dyes such as SYBR Green I (SGI) and thioflavin T (ThT) to perform label-free detection of aptamer binding has been performed for a long time for both binding assays and biosensor development.… read more here.
Keywords: label free; triton 100; surfactant assisted; signal stability ... See more keywords
Addressing the challenges of biomarker calibration standards in ligand-binding assays: a European Bioanalysis Forum perspective.
Sign Up to like & getrecommendations! Published in 2017 at "Bioanalysis"
DOI: 10.4155/bio-2017-0141
Abstract: The analysis of biomarkers by ligand-binding assays offers significant challenges compared with the bioanalysis of small and large molecule drugs. The presence of endogenous analyte is a commonly cited issue. Also the sourcing and application… read more here.
Keywords: biomarker; european bioanalysis; ligand binding; calibration ... See more keywords