The binacle (binding and cleavage) assay for in vitro determination of botulinum neurotoxin activity
Sign Up to like & getrecommendations! Published in 2018 at "Toxicon"
DOI: 10.1016/j.toxicon.2018.11.026
Abstract: Principle of the BINACLE assay. (I.) BoNT molecules (grey) bind to immobilized receptors (blue and red) via their H-chains. Then the L-chains are released by reduction of the disulfide bonds. (II.) The supernatant with the… read more here.
Keywords: binding cleavage; cleavage; binacle binding; binacle ... See more keywords
Dynamics of Target DNA Binding and Cleavage by Staphylococcus aureus Cas9 as Revealed by High-Speed Atomic Force Microscopy.
Sign Up to like & getrecommendations! Published in 2023 at "ACS nano"
DOI: 10.1021/acsnano.2c10709
Abstract: Programmable DNA binding and cleavage by CRISPR-Cas9 has revolutionized the life sciences. However, the off-target cleavage observed in DNA sequences with some homology to the target still represents a major limitation for a more widespread… read more here.
Keywords: cleavage; microscopy; dna binding; cas9 ... See more keywords
Cation assisted binding and cleavage of dinitrogen by uranium complexes
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DOI: 10.1039/d2sc02530b
Abstract: The role of alkali promoters in N2 cleavage by metal complexes remains poorly understood despite its relevance to the industrial production of ammonia from N2. Here we report a series of alkali bound-oxo-bridged diuranium(iii) complexes… read more here.
Keywords: cleavage; cation assisted; assisted binding; cation ... See more keywords