Affinities and Kinetics Detection of Protein-Small Molecule Interactions with a Monolayer MoS2 -Based Optical Imaging Platform.
Sign Up to like & getrecommendations! Published in 2022 at "Small"
DOI: 10.1002/smll.202202622
Abstract: Quantifying the binding kinetics and affinities of protein-small molecule interactions is critical for biomarker validation, drug discovery, and deep understanding of various biological processes at the molecular-scale. Novel approaches are demanded as most common label-free… read more here.
Keywords: small molecule; binding kinetics; molecule; molecule interactions ... See more keywords
Multi-scale modeling of drug binding kinetics to predict drug efficacy
Sign Up to like & getrecommendations! Published in 2019 at "Cellular and Molecular Life Sciences"
DOI: 10.1007/s00018-019-03376-y
Abstract: Optimizing drug therapies for any disease requires a solid understanding of pharmacokinetics (the drug concentration at a given time point in different body compartments) and pharmacodynamics (the effect a drug has at a given concentration).… read more here.
Keywords: drug binding; drug; binding kinetics; drug efficacy ... See more keywords
Exploring Ligand-Binding Kinetics in the S2 Site of MhsT by Atomistic Simulations and Markov Models
Sign Up to like & getrecommendations! Published in 2017 at "Biophysical Journal"
DOI: 10.1016/j.bpj.2016.11.711
Abstract: Neurotransmitter:Sodium Symporters (NSS) terminate neurotransmission through sodium-driven reuptake of cognate neurotransmitters, and traverse between outward-open and inward-open states. NSS include serotonin and dopamine transporters, which are targets for antidepressants and abused psychostimulants. Crystallographically, whereas both… read more here.
Keywords: ligand binding; binding kinetics; site; conformational changes ... See more keywords
Binding Kinetics and Lateral Mobility of HSV-1 on End-Grafted Sulfated Glycosaminoglycans.
Sign Up to like & getrecommendations! Published in 2017 at "Biophysical journal"
DOI: 10.1016/j.bpj.2017.06.028
Abstract: Many viruses, including herpes simplex (HSV), are recruited to their host cells via interaction between their envelope glycoproteins and cell-surface glycosaminoglycans (GAGs). This initial attachment is of a multivalent nature, i.e., it requires the establishment… read more here.
Keywords: lateral mobility; mobility; gag; binding kinetics ... See more keywords
Ligand binding kinetics at histamine H3 receptors by fluorescence‐polarization with real‐time monitoring
Sign Up to like & getrecommendations! Published in 2019 at "European Journal of Pharmacology"
DOI: 10.1016/j.ejphar.2019.01.041
Abstract: &NA; Growing evidence recommends incorporating the concept of drug‐target residence times within drug development and screening programs. For many targets, systematic research for binding kinetics is emerging and reported, as in case of the histamine… read more here.
Keywords: binding kinetics; ligand; fluorescence polarization; real time ... See more keywords
Exopolysaccharide from Streptococcus thermophilus as stabilizer in fermented dairy: Binding kinetics and interactions with casein of milk.
Sign Up to like & getrecommendations! Published in 2019 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2019.08.146
Abstract: Exopolysaccharides (EPSs) from lactic acid bacteria have great effect on the quality of fermented milk products. However, the mechanism for the quality improvement has not been well described. This study aimed to investigate the molecular… read more here.
Keywords: milk; casein milk; binding kinetics; acidifying process ... See more keywords
Dissecting the impact of target-binding kinetics of protein binders on tumor localization
Sign Up to like & getrecommendations! Published in 2021 at "iScience"
DOI: 10.1016/j.isci.2021.102104
Abstract: Summary Systematic control of in vivo behavior of protein-based therapeutics is considered highly desirable for improving their clinical outcomes. Modulation of biochemical properties including molecular weight, surface charge, and binding affinity has thus been suggested… read more here.
Keywords: protein binders; affinity; binding kinetics; tumor localization ... See more keywords
Quantifying Antibody Binding Kinetics on Fixed Cells and Tissues via Fluorescence Lifetime Imaging.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c01076
Abstract: We present a method for monitoring spatially localized antigen-antibody binding events on physiologically relevant substrates (cell and tissue sections) using fluorescence lifetime imaging. Specifically, we use the difference between the fluorescence decay times of fluorescently… read more here.
Keywords: binding kinetics; fluorescence lifetime; antibody binding; lifetime imaging ... See more keywords
New Methodology for Determining Plasma Protein Binding Kinetics Using an Enzyme Reporter Assay Coupling with High-Resolution Mass Spectrometry.
Sign Up to like & getrecommendations! Published in 2023 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c04864
Abstract: Determination of drug binding kinetics in plasma is important yet extremely challenging. Accordingly, we introduce "dynamic free fraction" as a new binding parameter describing drug-protein binding kinetics. We demonstrate theoretically and experimentally that the dynamic… read more here.
Keywords: protein binding; binding kinetics; methodology; fraction ... See more keywords
Reshaping the Energy Landscape Transforms the Mechanism and Binding Kinetics of DNA Threading Intercalation.
Sign Up to like & getrecommendations! Published in 2018 at "Biochemistry"
DOI: 10.1021/acs.biochem.7b01036
Abstract: Molecules that bind DNA via threading intercalation show high binding affinity as well as slow dissociation kinetics, properties ideal for the development of anticancer drugs. To this end, it is critical to identify the specific… read more here.
Keywords: dna threading; mechanism; dna; binding kinetics ... See more keywords
Kinetic Aspects of the Interaction between Ligand and G Protein-Coupled Receptor: The Case of the Adenosine Receptors.
Sign Up to like & getrecommendations! Published in 2017 at "Chemical reviews"
DOI: 10.1021/acs.chemrev.6b00025
Abstract: Ligand-receptor binding kinetics is an emerging topic in the drug research community. Over the past years, medicinal chemistry approaches from a kinetic perspective have been increasingly applied to G protein-coupled receptors including the adenosine receptors… read more here.
Keywords: binding kinetics; protein coupled; adenosine receptors; kinetic aspects ... See more keywords