Evaluation of preferred binding regions on ubiquitin and IgG1 FC for interacting with multimodal cation exchange resins using DEPC labeling/mass spectrometry
Sign Up to like & getrecommendations! Published in 2023 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.28361
Abstract: There is significant interest in identifying the preferred binding domains of biological products to various chromatographic materials. In this work, we develop a biophysical technique that uses diethyl pyrocarbonate (DEPC) based covalent labeling in concert… read more here.
Keywords: cation exchange; binding regions; preferred binding; depc labeling ... See more keywords
Computational Prediction of MoRFs, Short Disorder-to-order Transitioning Protein Binding Regions
Sign Up to like & getrecommendations! Published in 2019 at "Computational and Structural Biotechnology Journal"
DOI: 10.1016/j.csbj.2019.03.013
Abstract: Molecular recognition features (MoRFs) are short protein-binding regions that undergo disorder-to-order transitions (induced folding) upon binding protein partners. These regions are abundant in nature and can be predicted from protein sequences based on their distinctive… read more here.
Keywords: protein; protein binding; disorder order; morfs short ... See more keywords
CLIP: accurate prediction of disordered linear interacting peptides from protein sequences using co-evolutionary information
Sign Up to like & getrecommendations! Published in 2022 at "Briefings in bioinformatics"
DOI: 10.1093/bib/bbac502
Abstract: One of key features of intrinsically disordered regions (IDRs) is facilitation of protein-protein and protein-nucleic acids interactions. These disordered binding regions include molecular recognition features (MoRFs), short linear motifs (SLiMs) and longer binding domains. Vast… read more here.
Keywords: disordered protein; evolutionary information; binding regions; protein binding ... See more keywords
Structural and Biological Characterizations of Novel High-Affinity Fluorescent Probes with Overlapped and Distinctive Binding Regions on CXCR4
Sign Up to like & getrecommendations! Published in 2019 at "Molecules"
DOI: 10.3390/molecules24162928
Abstract: CXC-type chemokine receptor 4 (CXCR4) is well known as a co-receptor for cellular entry and infection of human immunodeficiency virus type 1 (HIV-1). As an important member of the G protein-coupled receptor (GPCR) family, CXCR4… read more here.
Keywords: high affinity; binding; binding regions; fluorescent probes ... See more keywords